Brown Brian D, Naldini Luigi
Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, 1425 Madison Avenue, BOX 1498, New York, New York 10029, USA.
Nat Rev Genet. 2009 Aug;10(8):578-85. doi: 10.1038/nrg2628.
New technologies are emerging that utilize artificial microRNA (miRNA) target sites to exploit or inhibit endogenous miRNA regulation. This approach has been used to improve cell-specific targeting for gene and stem cell therapy studies and for animal transgenics, and also to reduce the toxicity of oncolytic viruses and to attenuate viral vaccines. Artificial targets have also been used to sponge or decoy miRNAs as a way to study their functions. This article considers the benefits of this approach and design considerations for future studies.
利用人工微小RNA（miRNA）靶位点来开发或抑制内源性miRNA调控的新技术正在不断涌现。这种方法已被用于改善基因和干细胞治疗研究以及动物转基因研究中的细胞特异性靶向，还可降低溶瘤病毒的毒性并减弱病毒疫苗的效力。人工靶标也被用于吸附或诱捕miRNA，以此来研究它们的功能。本文探讨了这种方法的优势以及未来研究的设计考量。
