Cagan R H
J Neurosci Res. 1976;2(5-6):363-71. doi: 10.1002/jnr.490020504.
Labeling of cyclic AMP of tase papillae and its responsiveness to tast stimuli has been measured using whole papillae from bovine tongue prelabeled with [ 8-(14) C] adenine. Labeling was measured in circumvallate and fungiform papillae, both of which contain taste buds, and in filiform papillae and small blocks of tongue epithelium, which are devoid of taste buds. No differences were observed in the levels of activity. The labeling of cyclic AMP of circumvallate papillae showed only small increases (12-22%) in the presence of the taste stimulus sucrose (sweet), and the stimulatory effects were not statistically significant. The increase due to sucrose was not potentiated by theophylline. No stimulation by sucrose was observed with epithelium controls. Lactose, which is a poor taste stimulus, did not stimulate labeling of cyclic AMP in taste papillae. Theophylline, caffeine, and quinine (bitter) stimulated labeling of cyclic AMP by up to 2-fold, as did L-Epinephrine. Evidence for a specific role of cyclic AMP as a second messenger in taste sensation was not obtained. It is suggested that cyclic AMP might provide a mechanistic basis for studying some of the effects of sweet and bitter compounds in mixtures.
已使用预先用[8-(14)C]腺嘌呤标记的牛舌全乳头测量了味蕾乳头中环状AMP的标记及其对味觉刺激的反应性。在含有味蕾的轮廓乳头和菌状乳头以及不含味蕾的丝状乳头和小块舌上皮中测量了标记。未观察到活性水平的差异。在味觉刺激蔗糖（甜味）存在的情况下，轮廓乳头中环状AMP的标记仅略有增加（12 - 22%），且刺激作用无统计学意义。蔗糖引起的增加未被茶碱增强。上皮对照组未观察到蔗糖的刺激作用。作为不良味觉刺激物的乳糖未刺激味蕾乳头中环状AMP的标记。茶碱、咖啡因和奎宁（苦味）以及L - 肾上腺素可将环状AMP的标记刺激高达2倍。未获得环状AMP作为味觉感觉中第二信使的特定作用的证据。有人提出，环状AMP可能为研究甜味和苦味化合物在混合物中的某些作用提供一个机制基础。
