Yamamoto Y L, Ueda T, Shimauchi M, Diksic M
Neuroisotope Laboratory, Montreal Neurological Institute, Quebec, Canada.
Neurosurgery. 1991 Nov;29(5):719-25; discussion 725-6. doi: 10.1097/00006123-199111000-00013.
We examined the efficacy of a bypass from an extracerebral artery to a cerebral vein (EA-CV) with retrograde verapamil infusion on acute focal cerebral ischemia in 35 rats. In 12 rats, within 1 hour after occlusion of the middle cerebral artery (MCAO), changes in blood-brain barrier permeability were examined by [14C]alpha-aminoisobutyric acid autoradiography after EA-CV bypass surgery; there were no significant changes during a period of 2 hours after EA-CV bypass. The other 18 rats having MCAO were divided into three groups of six each. Group A rats (control) underwent only cannulation of the cerebral vein. Group B rats had an EA-CV bypass. Group C rats received verapamil (0.1 mg/kg every 2 h) by transvenous perfusion of the brain (TVPOB) through the EA-CV bypass. In all rats in Groups A and C, local cerebral blood flow (LCBF) and quantitative measurement of early cerebral infarct volume were performed by autoradiography using [14C]iodoantipyrine and histochemical staining methods. Group B rats were examined only with the LCBF measurement. Group B (EA-CV bypass only) showed a nonsignificant improvement (18-40%) of LCBF in the ischemic cerebral cortical areas as compared with control Group A. Group C (EA-CV bypass with TVPOB with verapamil) showed an extensive and significant improvement in LCBF in the ischemic cortical areas (115-140%; P less than 0.05) and a slight increase in LCBF in the subcortical areas (17-29%), with a significant reduction (greater than 35%; P less than 0.05) in a total cerebral infarct volume in the ischemic cerebral hemisphere as compared with the control Group A.(ABSTRACT TRUNCATED AT 250 WORDS)
我们研究了经脑外动脉至脑静脉搭桥术（EA-CV）并逆行输注维拉帕米对35只大鼠急性局灶性脑缺血的疗效。12只大鼠在大脑中动脉闭塞（MCAO）后1小时内，经EA-CV搭桥手术后，通过[14C]α-氨基异丁酸放射自显影术检测血脑屏障通透性的变化；EA-CV搭桥术后2小时内无显著变化。另外18只患有MCAO的大鼠被分为三组，每组6只。A组大鼠（对照组）仅进行脑静脉插管。B组大鼠进行EA-CV搭桥术。C组大鼠通过EA-CV搭桥经静脉脑灌注（TVPOB）接受维拉帕米（每2小时0.1mg/kg）。A组和C组的所有大鼠，通过[14C]碘代安替比林放射自显影术和组织化学染色方法进行局部脑血流量（LCBF）测定和早期脑梗死体积的定量测量。B组大鼠仅进行LCBF测量。与对照组A相比，B组（仅EA-CV搭桥术）在缺血性脑皮质区域的LCBF有不显著的改善（18 - 40%）。C组（EA-CV搭桥术联合TVPOB给予维拉帕米）在缺血性皮质区域的LCBF有广泛且显著的改善（115 - 140%；P < 0.05），在皮质下区域的LCBF略有增加（17 - 29%），与对照组A相比，缺血性脑半球的全脑梗死体积显著减少（大于35%；P < 0.05）。（摘要截取自250字）
