Ueda T, Yamamoto Y L, Diksic M
Cone Neurosurgical Research Laboratory, Montreal Neurological Institute, McGill University, Canada.
Stroke. 1989 Apr;20(4):501-6. doi: 10.1161/01.str.20.4.501.
We report on the effect of calcium channel blocker verapamil administered into the inferior cerebral vein in rats 1 hour after occlusion of the middle cerebral artery. Twenty-four rats were divided into four groups of six rats each. Group A rats received no medication. The other three groups received 0.1 mg verapamil/kg/2 hr. Group B rats received verapamil intravenously. Group C and D rats received verapamil and autologous arterial blood by transvenous perfusion of the brain, Group C rats at 100 mm Hg perfusion pressure and Group D rats at 150 mm Hg perfusion pressure. The administration of verapamil started 1 hour after middle cerebral artery occlusion and lasted for 2 hours. Three hours after occlusion, we used double- or single-tracer autoradiography with 4-[18F]fluoroantipyrine or [14C]iodoantipyrine and [14C]alpha-aminoisobutyric acid as tracers to study the brains for local cerebral blood flow and blood-brain barrier permeability changes. Group C showed a significant increase of local cerebral blood flow in the parietal cortex (89%, p less than 0.01) and sensorimotor cortex (64%, p less than 0.05) compared with Group A. Group D showed an extensive and striking increase in local cerebral blood flow of the ischemic cortical and subcortical areas (57-100%, p less than 0.05). Group B showed no significant changes but exhibited further reduction of local cerebral blood flow in the ischemic cerebral hemisphere associated with slightly increased local cerebral blood flow in the nonischemic cerebral hemisphere compared with Group A. There was no change of blood-brain barrier permeability in any group.(ABSTRACT TRUNCATED AT 250 WORDS)
我们报告了在大鼠大脑中动脉闭塞1小时后，将钙通道阻滞剂维拉帕米注入大脑下静脉的效果。24只大鼠被分为四组，每组6只。A组大鼠未接受药物治疗。其他三组每2小时接受0.1毫克/千克的维拉帕米。B组大鼠静脉注射维拉帕米。C组和D组大鼠通过经静脉灌注大脑接受维拉帕米和自体动脉血，C组大鼠灌注压力为100毫米汞柱，D组大鼠灌注压力为150毫米汞柱。维拉帕米在大脑中动脉闭塞1小时后开始给药，持续2小时。闭塞3小时后，我们使用4-[18F]氟安替比林或[14C]碘安替比林以及[14C]α-氨基异丁酸作为示踪剂进行双示踪或单示踪放射自显影，以研究大脑局部脑血流量和血脑屏障通透性的变化。与A组相比，C组顶叶皮质（增加89%，p<0.01）和感觉运动皮质（增加64%，p<0.05）的局部脑血流量显著增加。D组缺血皮质和皮质下区域的局部脑血流量广泛且显著增加（57-100%，p<0.05）。B组无显著变化，但与A组相比，缺血脑半球的局部脑血流量进一步减少，非缺血脑半球的局部脑血流量略有增加。任何一组的血脑屏障通透性均无变化。（摘要截断于250字）
