用脂质膜修饰电极以研究醌氧化还原酶。
Electrodes modified with lipid membranes to study quinone oxidoreductases.
作者信息
Weiss Sophie A, Jeuken Lars J C
机构信息
School of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, UK.
出版信息
Biochem Soc Trans. 2009 Aug;37(Pt 4):707-12. doi: 10.1042/BST0370707.
Abstract
Quinone oxidoreductases are a class of membrane enzymes that catalyse the oxidation or reduction of membrane-bound quinols/quinones. The conversion of quinone/quinol by these enzymes is difficult to study because of the hydrophobic nature of the enzymes and their substrates. We describe some biochemical properties of quinones and quinone oxidoreductases and then look in more detail at two model membranes that can be used to study quinone oxidoreductases in a native-like membrane environment with their native lipophilic quinone substrates. The results obtained with these model membranes are compared with classical enzyme assays that use water-soluble quinone analogues.
摘要
醌氧化还原酶是一类膜酶，可催化膜结合的对苯二酚/醌的氧化或还原。由于这些酶及其底物具有疏水性，因此很难研究这些酶对醌/对苯二酚的转化。我们描述了醌和醌氧化还原酶的一些生化特性，然后更详细地研究了两种模型膜，它们可用于在类似天然膜的环境中，与天然亲脂性醌底物一起研究醌氧化还原酶。将这些模型膜获得的结果与使用水溶性醌类似物的经典酶分析方法进行了比较。
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