Cooper Wendy A, Kohonen-Corish Maija R J, McCaughan Brian, Kennedy Catherine, Sutherland Robert L, Lee Cheok Soon
Department of Anatomical Pathology, Royal Prince Alfred Hospital, Sydney, NSW, Australia.
Histopathology. 2009 Jul;55(1):28-36. doi: 10.1111/j.1365-2559.2009.03331.x.
Aberrant expression of cell cycle regulators has been implicated in the pathogenesis of many neoplasms, including non-small cell lung cancer (NSCLC). The aim was to examine the expression and prognostic value of cyclin B1 and cyclin A, key regulators of the G(2)/M checkpoint of the cell cycle, in NSCLC and bronchial precursor lesions.
Immunohistochemical expression of cyclin B1 and A was examined in 90 cases of stage I-II primary NSCLC and bronchial precursor lesions using tissue microarrays. Increased cyclin B1 and A expression was found in 40.9 and 58.9% of NSCLC cases, respectively, and was significantly higher in primary NSCLC, lymph node metastases and some bronchial precursor lesions compared with normal bronchial epithelium. Increased expression of cyclin A and cyclin B1 correlated with tumour type, poorly differentiated tumours and male gender. A significant association was found between increased cyclin B1 expression and reduced survival using Kaplan-Meier survival analysis. On multivariate analysis, cyclin B1 was not an independent prognostic factor (P = 0.067). Cyclin A expression was not associated with survival.
Cyclin B1 and cyclin A are aberrantly expressed in NSCLC and some precursor lesions. Cyclin B1, but not cyclin A, shows some promise as a potential prognostic marker in NSCLC.
细胞周期调节因子的异常表达与包括非小细胞肺癌（NSCLC）在内的多种肿瘤的发病机制有关。本研究旨在检测细胞周期G(2)/M期关键调节因子细胞周期蛋白B1（cyclin B1）和细胞周期蛋白A（cyclin A）在NSCLC及支气管前驱病变中的表达及其预后价值。
采用组织芯片检测90例I-II期原发性NSCLC及支气管前驱病变中cyclin B1和cyclin A的免疫组化表达。NSCLC病例中cyclin B1和cyclin A表达增加的分别占40.9%和58.9%，与正常支气管上皮相比，原发性NSCLC、淋巴结转移灶及部分支气管前驱病变中二者表达显著更高。cyclin A和cyclin B1表达增加与肿瘤类型、低分化肿瘤及男性性别相关。采用Kaplan-Meier生存分析发现cyclin B1表达增加与生存率降低显著相关。多因素分析显示，cyclin B1不是独立的预后因素（P = 0.067）。cyclin A表达与生存率无关。
cyclin B1和cyclin A在NSCLC及部分前驱病变中异常表达。cyclin B1而非cyclin A有望作为NSCLC潜在的预后标志物。
