Fei Li-ming, Wang Chang-liang, Zhao Wen-hua, Cui Kai, Zhang Bo, Zhou Wu-yuan, Zhong Wei-xia, Li Sheng
Department of Hepatobiliary Surgery, Yantaishan Hospital, Yantai 264001, China.
Zhonghua Wai Ke Za Zhi. 2009 May 15;47(10):783-6.
To investigate the expression of CXCL12, its receptor CXCR4 and its correlations with clinical pathology and lymphangiogenesis in pancreatic adenocarcinoma (PAC).
The tissue samples were obtained from 30 patients with PAC by surgery between January 2005 and December 2007, which including PAC, the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lymph nodes. The patients age ranged from 35 to 78 years old (median 57.2 years old). The expressions of CXCL12 and CXCR4 in these tissues were assayed by immunohistochemical staining, RT-PCR and fluorescence quantitative real-time PCR.
In the immunohistochemical staining, the CXCL12 protein mainly located in the normal pancreatic cell envelopes and/or cytolymphs. In the immunohistochemical staining, the CXCR4 protein mainly located in the cell envelopes and/or cytolymphs of PAC. The results of RT-PCR and fluorescence quantitative real-time PCR indicated that the expression levels of CXCR4 mRNA in PAC tissues, the cancerous peripheral tissues and peripheral lympho nodes were higher than that in the normal pancreatic tissues (P < 0.01). The MLVD in PAC were detected by morphometric analysis respectively. The level of MLVD in III-IV stages was higher than I-II stages of PAC (P < 0.01), and in these cases which had lymphatic metastasis, the level of MLVD significantly increased (P < 0.01). And there was no correlation between the differentiation and histology types of PAC (P > 0.05). There was 22 samples that the CXCR4 protein was positive, and among these samples the MLVD was higher than that in negative group of CXCR4 protein (P = 0.003).
The expression of CXCR4 was significantly associated with lymphatic metastasis of PAC, and the higher expression of CXCR4 in PAC tissues was significantly associated with lymphangiogenesis of PAC.
探讨趋化因子CXCL12及其受体CXCR4在胰腺腺癌（PAC）中的表达情况，以及它们与临床病理特征和淋巴管生成的相关性。
收集2005年1月至2007年12月间30例接受手术治疗的PAC患者的组织样本，包括PAC组织、癌旁组织、正常胰腺组织及外周淋巴结。患者年龄35～78岁（中位年龄57.2岁）。采用免疫组织化学染色、逆转录-聚合酶链反应（RT-PCR）及荧光定量实时PCR检测上述组织中CXCL12和CXCR4的表达。
免疫组织化学染色显示，CXCL12蛋白主要位于正常胰腺细胞包膜及/或细胞浆内。CXCR4蛋白主要位于PAC细胞包膜及/或细胞浆内。RT-PCR及荧光定量实时PCR结果显示，PAC组织、癌旁组织及外周淋巴结中CXCR4 mRNA表达水平高于正常胰腺组织（P<0.01）。分别采用形态计量学分析检测PAC中的微淋巴管密度（MLVD）。PACⅢ-Ⅳ期的MLVD水平高于Ⅰ-Ⅱ期（P<0.01），有淋巴结转移者MLVD水平显著升高（P<0.01）。PAC的分化程度和组织学类型之间无相关性（P>0.05）。CXCR4蛋白阳性的样本有22例，这些样本的MLVD高于CXCR4蛋白阴性组（P=0.003）。
CXCR4的表达与PAC的淋巴结转移显著相关，PAC组织中CXCR4的高表达与PAC的淋巴管生成显著相关。
