Yan Chang-qing, Zhao Yu-pei
Department of Hepatobiliary Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050011, China.
Zhonghua Wai Ke Za Zhi. 2009 May 15;47(10):787-90.
To investigate the relationship between expression of angiogenic factors and invasion and proliferation in pancreatic carcinoma cell.
Expressions and secretions of angiogenic factors in pancreatic carcinoma cell lines were determined by RT-PCR and ELISA. Proliferation and invasion of pancreatic carcinoma cell lines were determined by CCK-8 and Boyden Chamber invasion tests.
Pancreatic carcinoma cell lines showed significantly different ability of invasion and proliferation. Intracellular VEGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (152.9 +/- 0.6), (224.1 +/- 60.3), (239.2 +/- 2.1), (19.3 +/- 0.7), (165.6 +/- 34.3), and (18.1 +/- 1.4) pg/(10(6)cell.24 h). Extracellular VEGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (1331.1 +/- 67.8), (3902.6 +/- 79.7), (2657.3 +/- 51.9), (1498.3 +/- 4.8), (4696.8 +/- 45.5), and (1200.5 +/- 42.2) pg/(10(6)cell.24 h). Intracellular bFGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (66.1 +/- 4.8), (206.8 +/- 99.5), (1532.0 +/- 54.6), (159.2 +/- 11.0), (1612.0 +/- 515.9) and (2781.2 +/- 479.0) pg/(10(6)cell.24 h). Extracellular bFGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (2.1 +/- 0.6), (10.3 +/- 1.5), (31.0 +/- 0.4), (4.3 +/- 1.2), (43.6 +/- 1.5) and (82.1 +/- 10.4) pg/(10(6)cell.24 h). Intracellular endostatin expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (0.2 +/- 0.0), (0.3 +/- 0.0), (4.7 +/- 0.1), (10.8 +/- 0.2), (31.9 +/- 11.7) and (5.4 +/- 0.1) ng/(10(6)cell.24 h). Extracellular endostatin expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (0.0 +/- 0.0), (1.6 +/- 0.0), (21.5 +/- 1.1), (40.8 +/- 0.4), (129.2 +/- 1.0) and (20.1 +/- 1.8) ng/(10(6)cell.24 h). Panc-1 enjoying stronger invasion and proliferation showed stronger expressions of VEGF, bFGF and endostatin. Intracellular expressions of bFGF was stronger than extracellular, extracellular expressions of VEGF and endostatin were stronger than intracellular.
Expressions of angiogenic factors regulated by cancer cell played an important role in progression of pancreatic carcinoma.
探讨血管生成因子表达与胰腺癌细胞侵袭和增殖之间的关系。
采用逆转录-聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）法检测胰腺癌细胞系中血管生成因子的表达及分泌情况。采用细胞计数试剂盒-8（CCK-8）法和Boyden小室侵袭试验检测胰腺癌细胞系的增殖和侵袭能力。
胰腺癌细胞系的侵袭和增殖能力存在显著差异。SW1990、Capan-1、Aspc-1、MiaPaCa-2、Panc-1和PCT-3细胞系的细胞内血管内皮生长因子（VEGF）表达分别为（152.9±0.6）、（224.1±60.3）、（239.2±2.1）、（19.3±0.7）、（165.6±34.3）和（18.1±1.4）pg/（10⁶细胞·24小时）。SW1990、Capan-1、Aspc-1、MiaPaCa-2、Panc-1和PCT-3细胞系的细胞外VEGF表达分别为（1331.1±67.8）、（3902.6±79.7）、（
