Jiang Hua, Zhao Yu-pei, Chen Ge, Wu Wei-wei, Zhang Fu-quan, Dong Yu-xing, Wu Yuan-de
Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.
Zhonghua Wai Ke Za Zhi. 2006 Dec 1;44(23):1640-3.
To explore the method of inducing and building pancreatic cancer cell sublines with radiation resistance.
Simulating the clinical radiotherapy, the pancreatic cell lines SW1990, Capan-1 (Cap), AsPC-1 (ASPC), P3, PANC-1 (Pan-1) and MIAPaCa-2 (MIA) were repeatedly given individual dose of X-rays with liner accelerator to induce radiation resistance, the changes of cell morphology, cell cycle and radio sensibility in the induced cell lines were compared with the parental cell lines at the end of inducing course.
Compared with the parental cells, there were significant changes in morphology in the pancreatic cancer cell sublines after the radiation. Cell cycle analysis suggested that SW1990-R, ASPC-R, MIA-R, PAN-R and P3-R had lower G(2)/M and greater SF(2) (survival fraction after 2 Gy irradiation) compared with the parental cell lines.
The method of radiating cells step by step and repeatedly is viable to establish radio-resistant pancreatic cancer cell lines.
探索诱导并构建具有辐射抗性的胰腺癌细胞亚系的方法。
模拟临床放射治疗，使用直线加速器对胰腺癌细胞系SW1990、Capan-1（Cap）、AsPC-1（ASPC）、P3、PANC-1（Pan-1）和MIAPaCa-2（MIA）反复给予单次剂量的X射线以诱导辐射抗性，在诱导过程结束时，将诱导细胞系中的细胞形态、细胞周期和放射敏感性的变化与亲代细胞系进行比较。
与亲代细胞相比，辐射后胰腺癌细胞亚系的形态有显著变化。细胞周期分析表明，与亲代细胞系相比，SW1990-R、ASPC-R、MIA-R、PAN-R和P3-R的G(2)/M期较低，2 Gy照射后的存活分数（SF(2)）较高。
逐步反复照射细胞的方法对于建立抗辐射胰腺癌细胞系是可行的。
