硝酸在石墨炉原子吸收光谱法测定血清中痕量锂时的优越性。

Superiority of nitric acid for deproteinization in the determination of trace lithium in serum by graphite furnace atomic absorption spectrometry.

机构信息

Shanghai Key Laboratory of Vascular Biology at Ruijin Hospital and Shanghai Institute of Hypertension, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai 200025, China.

出版信息

J Pharm Biomed Anal. 2009 Dec 5;50(5):1075-9. doi: 10.1016/j.jpba.2009.06.044. Epub 2009 Jul 2.

DOI:10.1016/j.jpba.2009.06.044

PMID:19616397

Abstract

Physiological level of trace lithium in human serum was determined by graphite furnace atomic absorption spectrometry (GFAAS). 3.5% HNO3 (v/v) was employed as a protein precipitant for sample treatment and at the same time verified as a very effective chemical modifier to eliminate the interference of chloride. The analytical conditions for lithium determination in serum were investigated and the optimal pyrolysis and atomization temperatures were 800 degrees C and 2700 degrees C. The accuracy and precision of the method were tested by determining lithium in a RANDOX HN1530 assayed human multi-sera and a pooled human serum. The result was in good agreement with the target value and CV of the pooled human serum was 4.74% (n=10). The characteristic mass, the limit of detection (LOD) of the proposed method were 0.8 pg and 0.01 micromol/L, respectively. Median+/-S.E.M. of serum lithium in 220 Chinese people was 0.25+/-0.02 micromol/L.

摘要

采用石墨炉原子吸收光谱法（GFAAS）测定人血清中痕量锂的生理水平。3.5% HNO3（v/v）用作样品处理的蛋白质沉淀剂，同时被验证为一种非常有效的化学修饰剂，可消除氯离子的干扰。对血清中锂的分析条件进行了研究，最佳的热解和原子化温度分别为 800°C 和 2700°C。通过测定 RANDOX HN1530 分析的人多血清和混合人血清中的锂来检验方法的准确性和精密度。结果与混合人血清的目标值吻合良好，混合人血清的 CV 为 4.74%（n=10）。该方法的特征质量、检出限（LOD）分别为 0.8 pg 和 0.01 μmol/L。220 名中国人血清中锂的中位数±SEM 为 0.25±0.02 μmol/L。