即使在优化的实验条件下，在酵母线粒体中也能观察到钙离子诱导的通透性转变。
Ca2+-induced permeability transition can be observed even in yeast mitochondria under optimized experimental conditions.
作者信息
Yamada Akiko, Yamamoto Takenori, Yoshimura Yuya, Gouda Shunichi, Kawashima Satoshi, Yamazaki Naoshi, Yamashita Kikuji, Kataoka Masatoshi, Nagata Toshihiko, Terada Hiroshi, Pfeiffer Douglas R, Shinohara Yasuo
机构信息
School of Dentistry, University of Tokushima, Tokushima 770-8504, Japan.
出版信息
Biochim Biophys Acta. 2009 Dec;1787(12):1486-91. doi: 10.1016/j.bbabio.2009.07.001. Epub 2009 Jul 16.
Yeast mitochondria have generally been believed not to undergo the permeability transition (PT) by the accumulation of Ca(2+) within the mitochondrial matrix, unlike mammalian mitochondria. However, the reason why the yeast PT is not induced by Ca(2+) has remained obscure. In this study, we examined in detail the effects of Ca(2+) on yeast mitochondria under various conditions. As a result, we discovered that the PT could be induced even in yeast mitochondria by externally added Ca(2+) under optimized experimental conditions. The 2 essential parameters for proper observation of the PT-inducing effects of Ca(2+) were the concentrations of the respiratory substrate and that of inorganic phosphate (Pi) in the incubation medium. The yeast mitochondrial PT induced by Ca(2+) was found to be insensitive to cyclosporin A and suppressed in the presence of a high concentration of Pi. Furthermore, when the PT was induced in yeast mitochondria by Ca(2+), the release of cytochrome c from mitochondria was also observed.
与哺乳动物线粒体不同，一般认为酵母线粒体不会因线粒体基质内Ca(2+)的积累而发生通透性转换（PT）。然而，酵母PT不受Ca(2+)诱导的原因仍不清楚。在本研究中，我们详细研究了在各种条件下Ca(2+)对酵母线粒体的影响。结果发现，在优化的实验条件下，通过外部添加Ca(2+)，即使在酵母线粒体中也能诱导PT。正确观察Ca(2+)诱导PT效应的两个关键参数是呼吸底物的浓度和孵育培养基中无机磷酸盐（Pi）的浓度。发现由Ca(2+)诱导的酵母线粒体PT对环孢菌素A不敏感，并且在高浓度Pi存在下受到抑制。此外，当Ca(2+)在酵母线粒体中诱导PT时，还观察到细胞色素c从线粒体中释放。
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