Department of Biochemistry and Biotechnology, University of Thessaly, Larissa 41221, Greece.
Free Radic Biol Med. 2009 Nov 15;47(10):1371-4. doi: 10.1016/j.freeradbiomed.2009.07.014. Epub 2009 Jul 17.
This study investigated whether selected oxidative stress markers measured in blood adequately reflect redox status in skeletal muscle, heart, and liver. Several markers were determined after implementing two treatments known to affect redox status, namely exercise and allopurinol administration. Xanthine oxidase, thiobarbituric acid-reactive substances (TBARS), protein carbonyls (PC), reduced glutathione (GSH), oxidized glutathione (GSSG), catalase, and total antioxidant capacity were determined in blood, skeletal muscle, heart, and liver. Correlation between blood and tissues in each marker was performed through the Spearman rank correlation coefficient. GSSG in erythrocytes was correlated with all tissues, ranging in the five experimental groups as follows: skeletal muscle r(s)=0.656-0.874, heart r(s)=0.742-0.981, liver r(s)=0.646-0.855. Xanthine oxidase and TBARS measured in blood satisfactorily described the redox status of the heart (0.753-0.964 and 0.705-1.000, respectively) and liver (0.755-0.902 and 0.656-1.000, respectively). Skeletal muscle and heart redox status can be adequately described by PC (0.652-1.000 and 0.656-0.964, respectively), GSH (0.693-1.000 and 0.656-1.000, respectively), and catalase (0.745-1.000 and 0.656-1.000, respectively) measured in blood. In conclusion, this study suggests that a combination of markers measured in blood provides a reliable indication about the redox status in skeletal muscle, heart, and liver.
本研究旨在探讨血液中选定的氧化应激标志物是否能充分反映骨骼肌、心脏和肝脏的氧化还原状态。在实施两种已知影响氧化还原状态的处理后，即运动和别嘌呤醇给药，确定了几种标志物。在血液、骨骼肌、心脏和肝脏中测定黄嘌呤氧化酶、硫代巴比妥酸反应物质（TBARS）、蛋白质羰基（PC）、还原型谷胱甘肽（GSH）、氧化型谷胱甘肽（GSSG）、过氧化氢酶和总抗氧化能力。通过斯皮尔曼等级相关系数对每个标志物的血液和组织之间进行相关性分析。红细胞中的 GSSG 与所有组织相关，在五个实验组中的范围如下：骨骼肌 r(s)=0.656-0.874、心脏 r(s)=0.742-0.981、肝脏 r(s)=0.646-0.855。血液中测定的黄嘌呤氧化酶和 TBARS 能很好地描述心脏（0.753-0.964 和 0.705-1.000）和肝脏（0.755-0.902 和 0.656-1.000）的氧化还原状态。PC（0.652-1.000 和 0.656-0.964）、GSH（0.693-1.000 和 0.656-1.000）和血液中测定的过氧化氢酶（0.745-1.000 和 0.656-1.000）可充分描述骨骼肌和心脏的氧化还原状态。综上所述，本研究表明，血液中测定的标志物组合可提供关于骨骼肌、心脏和肝脏氧化还原状态的可靠指标。
