Biochemistry Unit, Regional Animal Health Research Laboratory, Animal Health Research Institute, Agriculture Research Center, Assiut, 71526, Egypt.
Department of Zoology, Faculty of Science, New Valley University, El-Kharga, 725211, Egypt.
Trop Anim Health Prod. 2022 Sep 10;54(5):288. doi: 10.1007/s11250-022-03284-7.
Trichophyton verrucosum is a zoophilic dermatophyte that causes skin inflammation. The present study aimed to evaluate the redox status in the blood of sheep clinically infected with T. verrucosum. According to clinical and mycological investigations, 48 juvenile male Balady sheep were selected in their natural habitat and divided into four groups depending on the lesion size: mild (MID), moderate (MOD), severe (SEV) T. verrucosum infection, and healthy control groups. Compared to the controls, plasma superoxide anion increased (P < 0.05) in both MOD and SEV but total peroxides (TPx) gradually increased (P < 0.05) in MID followed by MOD and SEV. Superoxide dismutase and total antioxidant capacity (TAC) were higher (P < 0.05) in MID and lower (P < 0.05) in MOD and SEV than in controls, but SEV showed lower TAC than MOD. Malondialdehyde (MDA, a lipid peroxide marker) increased (P < 0.05) in SEV than in controls, but protein carbonyl (PC, a protein peroxidation marker) was augmented (P < 0.05) as lesions progressed from mild to severe. The oxidative stress index (TPx/TAC ratio) progressively increased (P < 0.05) in MOD and SEV. The correlation of PC was positive with TPx and negative with TAC (P < 0.01). In conclusion, sheep infection with T. verrucosum is characterized by increased TPx and decreased TAC in plasma depending on the lesion area. The redox status is shifted towards the oxidizing state, particularly in MOD and SEV cases. This results in a condition of OS, which may contribute to the pathogenesis of the disease.
疣状毛癣菌是一种亲动物性的皮肤真菌，可引起皮肤炎症。本研究旨在评估临床感染疣状毛癣菌的绵羊血液中的氧化还原状态。根据临床和真菌学调查，在其自然栖息地选择了 48 只幼龄雄性 Balady 绵羊，并根据病变大小分为四组：轻度（MID）、中度（MOD）、重度（SEV）感染，以及健康对照组。与对照组相比，MOD 和 SEV 组的血浆中超氧阴离子增加（P<0.05），而 MID 组的总过氧化物（TPx）逐渐增加（P<0.05），随后是 MOD 和 SEV 组。与对照组相比，MID 组的超氧化物歧化酶和总抗氧化能力（TAC）较高（P<0.05），MOD 和 SEV 组较低（P<0.05），但 SEV 组的 TAC 低于 MOD 组。丙二醛（MDA，一种脂质过氧化物标志物）在 SEV 组中高于对照组（P<0.05），但随着病变从轻到重进展，蛋白质羰基（PC，一种蛋白质过氧化标志物）增加（P<0.05）。氧化应激指数（TPx/TAC 比值）在 MOD 和 SEV 组中逐渐增加（P<0.05）。PC 与 TPx 呈正相关，与 TAC 呈负相关（P<0.01）。总之，绵羊感染疣状毛癣菌后，根据病变面积，血浆中 TPx 增加，TAC 减少。氧化还原状态向氧化状态转移，特别是在 MOD 和 SEV 病例中。这导致 OS 状态，这可能有助于疾病的发病机制。
