Astuti Yeni, Topoglidis Emmanuel, Cass Anthony G, Durrant James R
Department of Chemistry, Imperial College, London SW7 2AY, United Kingdom.
Anal Chim Acta. 2009 Aug 19;648(1):2-6. doi: 10.1016/j.aca.2009.06.031. Epub 2009 Jun 21.
In this paper, we employ two peroxidases (horseradish peroxidase, HRP and cytochrome c peroxidase, CcP) to demonstrate their ability to retain their redox and biological functions after their immobilisation on mesoporous TiO(2) and SnO(2) electrodes. We will also demonstrate the use of HRP immobilised on the metal oxide electrodes for the development of reagentless optical and electrochemical biosensors for the detection of hydrogen peroxide (H(2)O(2)) with low detection limit of 0.04 and 1 microM, respectively.
在本文中，我们使用两种过氧化物酶（辣根过氧化物酶，HRP和细胞色素c过氧化物酶，CcP）来证明它们固定在介孔TiO(2)和SnO(2)电极上后保持其氧化还原和生物学功能的能力。我们还将展示固定在金属氧化物电极上的HRP用于开发无试剂光学和电化学生物传感器，用于检测过氧化氢（H(2)O(2)），其检测限分别低至0.04和1 microM。
