Department of Analytical Chemistry, Ghent University, Krijgslaan 281 S12, B-9000 Ghent, Belgium.
Bioelectrochemistry. 2012 Feb;83:15-8. doi: 10.1016/j.bioelechem.2011.07.001. Epub 2011 Aug 1.
A novel and versatile method, based on a membrane-free enzyme electrode in which both the enzyme and a mediator protein are entrapped in a gelatine hydrogel was developed for the fabrication of biosensors. As a proof of principle, we prepared a hydrogen peroxide biosensor by successfully entrapping both horse heart cytochrome c (HHC) and Saccharomyces cerevisae cytochrome c peroxidase (CCP) in a gelatin matrix which is immobilized on a gold electrode. This electrode was first pretreated with 6-mercaptohexanol. The biosensor displayed a rapid response and an expanded linear response range from 0 to 0.3 mM (R = 0.987) with a detection limit of 1 × 10(-5)M in a HEPES buffer solution (pH 7.0). This method of encapsulation is now further investigated for industrial biosensor applications.
一种新颖且通用的方法，基于一种无膜的酶电极，其中酶和介体蛋白都被包埋在明胶水凝胶中，用于生物传感器的制造。作为原理的证明，我们通过成功地将马心细胞色素 c（HHC）和酿酒酵母细胞色素 c 过氧化物酶（CCP）包埋在明胶基质中，制备了一种过氧化氢生物传感器，该基质固定在金电极上。该电极先用 6-巯基己醇预处理。在 HEPES 缓冲溶液（pH 7.0）中，该生物传感器在 0 至 0.3 mM 范围内表现出快速响应和扩展的线性响应范围（R = 0.987），检测限为 1×10（-5）M。这种包封方法现在进一步研究用于工业生物传感器应用。
