Chen Shujun, Kord Alireza
Analytical Sciences, Chemical Development, GlaxoSmithKline, King of Prussia, PA 19406, USA.
J Chromatogr A. 2009 Aug 21;1216(34):6204-9. doi: 10.1016/j.chroma.2009.06.084. Epub 2009 Jul 7.
Ultra performance liquid chromatography (UPLC) using small particles and very high pressure has demonstrated higher resolution and speed compared with conventional HPLC. An additional benefit of UPLC is the significantly reduced consumption of mobile phase. This report discusses how column length, particle size, inner column diameter, extra column void volume, and capacity factor contribute to the reduction of mobile phase consumption in UPLC compared with HPLC. In addition, theoretical and experimental comparison of mobile phase consumption was made between isocratic HPLC and UPLC as well as between gradient HPLC and UPLC. Both theoretical and experimental results indicate that UPLC typically saves at least 80% of mobile phase in isocratic and gradient conditions when compared with HPLC.
与传统高效液相色谱（HPLC）相比，使用小颗粒填料和超高压的超高效液相色谱（UPLC）具有更高的分离度和更快的分析速度。UPLC的另一个优点是流动相消耗显著降低。本报告讨论了与HPLC相比，柱长、粒径、柱内径、柱外死体积和容量因子如何有助于减少UPLC中的流动相消耗。此外，还对等度HPLC与UPLC以及梯度HPLC与UPLC之间的流动相消耗进行了理论和实验比较。理论和实验结果均表明，与HPLC相比，UPLC在等度和梯度条件下通常可节省至少80%的流动相。
