霍乱弧菌丝状噬菌体CTXphi和VGJphi的DNA结合蛋白
DNA binding proteins of the filamentous phages CTXphi and VGJphi of Vibrio cholerae.
作者信息
Falero Alina, Caballero Andy, Ferrán Beatriz, Izquierdo Yovanny, Fando Rafael, Campos Javier
机构信息
Department of Molecular Biology, National Center for Scientific Research, P.O. Box 6414, Ave. 25 y 158, Cubanacán, Playa, Ciudad de la Habana, Cuba.
出版信息
J Bacteriol. 2009 Sep;191(18):5873-6. doi: 10.1128/JB.01206-08. Epub 2009 Jul 17.
Abstract
The native product of open reading frame 112 (orf112) and a recombinant variant of the RstB protein, encoded by Vibrio cholerae pathogen-specific bacteriophages VGJphi and CTXphi, respectively, were purified to more than 90% homogeneity. Orf112 protein was shown to specifically bind single-stranded genomic DNA of VGJphi; however, RstB protein unexpectedly bound double-stranded DNA in addition to the single-stranded genomic DNA. The DNA binding properties of these proteins may explain their requirement for the rolling circle replication of the respective phages and RstB's requirement for single-stranded-DNA chromosomal integration of CTXphi phage dependent on XerCD recombinases.
摘要
霍乱弧菌病原体特异性噬菌体VGJphi和CTXphi分别编码的开放阅读框112（orf112）的天然产物和RstB蛋白的重组变体被纯化至均一性超过90%。已证明Orf112蛋白能特异性结合VGJphi的单链基因组DNA；然而，RstB蛋白除了能结合单链基因组DNA外，还意外地能结合双链DNA。这些蛋白质的DNA结合特性可能解释了它们对于各自噬菌体滚环复制的需求，以及RstB对于依赖XerCD重组酶的CTXphi噬菌体单链DNA染色体整合的需求。
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