霍乱弧菌O139丝状噬菌体（fsl）的分子分析
Molecular analysis of a filamentous phage (fsl) of Vibrio cholerae O139.
作者信息
Honma Y, Ikema M, Toma C, Ehara M, Iwanaga M
机构信息
Department of Bacteriology, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
出版信息
Biochim Biophys Acta. 1997 Dec 31;1362(2-3):109-15. doi: 10.1016/s0925-4439(97)00055-0.
A filamentous bacteriophage from Vibrio cholerae O139 strain A1-4450 was isolated (fsl). The phage fsl had a ssDNA genome and dsDNA as a replicative form (RF) in lysogenic host cell. The DNA sequence of fsl RF was determined. It consisted of 6340 bp and had a G + C content of 43.5%. Fifteen possible ORFs were found in fsl. One of them, ORF384, was estimated to encode 384 amino acid residues (44.6 kDa) and had homologous regions with the zot gene of V. cholerae and gene I of the coliphage group. ORF104, located upstream of ORF384, was homologous to gene 93 protein of Pf3 (filamentous phage of Pseudomonas sp.) corresponding to gene VI of coliphage. Other than ORF384 and ORF104, the ORF81, ORF44, ORF29, and ORF193 were speculated to correspond to gene V, gene VII, gene IX, and gene III, respectively, in the order as reported on f1 phage.
从霍乱弧菌O139菌株A1 - 4450中分离出一种丝状噬菌体（fsl）。噬菌体fsl具有单链DNA基因组，在溶原性宿主细胞中以双链DNA作为复制形式（RF）。测定了fsl RF的DNA序列。它由6340 bp组成，G + C含量为43.5%。在fsl中发现了15个可能的开放阅读框（ORF）。其中一个ORF384估计编码384个氨基酸残基（44.6 kDa），并与霍乱弧菌的zot基因和大肠杆菌噬菌体群的基因I具有同源区域。位于ORF384上游的ORF104与Pf3（假单胞菌属的丝状噬菌体）的基因93蛋白同源，对应于大肠杆菌噬菌体的基因VI。除了ORF384和ORF104外，推测ORF81、ORF44、ORF29和ORF193分别对应于f1噬菌体中报道的基因V、基因VII、基因IX和基因III。
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