Wan Ting, Liu Ji-Hong, Zheng Li-Min, Cai Mu-Yan, Ding Tong
State Key Laboratory of Oncology in South China, Guangzhou, Guangdong, P. R. China.
Ai Zheng. 2009 Mar;28(3):323-7.
Tumor-associated macrophage (TAM) infiltration promotes the progression of various malignancies. This study was to investigate the influence of TAM infiltration on the survival and prognosis of patients with ovarian cancer.
The expression of CD68, a TAM-specific marker, in 67 specimens of ovarian cancer and 22 specimens of benign ovarian lesion was detected by SP immunohistochemistry to investigate the density of TAM. The correlation of TAM density to the prognosis of ovarian cancer was analyzed. Cox multivariate proportional hazard model was used to analyze prognostic factors of ovarian cancer.
Observing under x 400 lens, the mean density of TAM was significantly higher in ovarian cancer than in benign ovarian lesions (57.7 vs. 25.3 per vision field, p<0.01). The 5-year survival rate was significantly higher in low-density TAM group than in high-density TAM group of ovarian cancer patients (73.3% vs. 41.2%, p=0.01). Univariate analysis found that TAM infiltration was more obvious in serous carcinoma, moderately and poorly differentiated carcinoma and in patients aged >or=40 than in the counterparts. Multivariate analysis revealed that histological grade and TAM infiltration status are independent predictors for overall survival.
TAM infiltration is obvious in advanced ovarian cancer, which indicates poor prognosis.
肿瘤相关巨噬细胞（TAM）浸润促进多种恶性肿瘤进展。本研究旨在探讨TAM浸润对卵巢癌患者生存及预后的影响。
采用SP免疫组化法检测67例卵巢癌标本及22例卵巢良性病变标本中TAM特异性标志物CD68的表达，以研究TAM密度。分析TAM密度与卵巢癌预后的相关性。采用Cox多因素比例风险模型分析卵巢癌的预后因素。
在400倍镜下观察，卵巢癌中TAM的平均密度显著高于卵巢良性病变（每视野57.7个 vs. 25.3个，p<0.01）。卵巢癌患者中，TAM低密度组的5年生存率显著高于TAM高密度组（73.3% vs. 41.2%，p=0.01）。单因素分析发现，浆液性癌、中低分化癌及年龄≥40岁患者的TAM浸润比相应患者更明显。多因素分析显示，组织学分级和TAM浸润状态是总生存的独立预测因素。
晚期卵巢癌中TAM浸润明显，提示预后不良。
