Department of Laboratory Medicine, Warren Magnuson Clinical Center, National Institutes of Health, Bethesda, MD 20892-1508, USA.
J Proteomics. 2010 Jan 3;73(3):629-36. doi: 10.1016/j.jprot.2009.07.001. Epub 2009 Jul 17.
One of the greatest challenges in analyzing the plasma proteome is the wide range of concentration of different proteins. The current study examines the range of protein concentration for 18 proteins measured over a year in a clinical laboratory to provide data on pathological extremes in protein concentrations. The complete measured range, from upper limits for albumin to lowest values for thyroid-stimulating hormone (TSH), represented more than 10 logs of molar abundance. A number of plasma proteins measured in the clinical laboratory varied over a concentration range spanning more than 4 logs, and limits of detection of clinical assays were inadequate to assess full concentration ranges of several proteins. Considering reported values from studies using higher sensitivity assays suggest that plasma concentrations of prostate-specific antigen (PSA), human chorionic gonadotropin (hCG), and cardiac troponin I vary by more than 7 logs. All of the plasma proteins measured in the present study represent secretory proteins or highly expressed components of specific tissues. Thus, the dynamic range for these components is likely to greatly underestimate the total range of protein concentration in the plasma proteome.
在分析血浆蛋白质组时，最大的挑战之一是不同蛋白质浓度范围很广。本研究检查了临床实验室中 18 种蛋白质在一年内测量的蛋白质浓度范围，为蛋白质浓度的病理极值提供了数据。从白蛋白的上限到促甲状腺激素（TSH）的最低值，完整的测量范围代表了超过 10 个摩尔丰度对数。临床实验室中测量的许多血浆蛋白在浓度范围上变化超过 4 个对数，临床测定的检测限不足以评估几种蛋白质的完整浓度范围。考虑到使用更高灵敏度测定法的研究报告值表明，前列腺特异性抗原（PSA）、人绒毛膜促性腺激素（hCG）和心肌肌钙蛋白 I 的血浆浓度变化超过 7 个对数。本研究中测量的所有血浆蛋白均代表分泌蛋白或特定组织中高度表达的成分。因此，这些成分的动态范围可能大大低估了血浆蛋白质组中蛋白质浓度的总范围。
