Department of Microsystems Engineering (IMTEK), Chemistry & Physics of Interfaces, University of Freiburg, Freiburg im Breisgau 79110, Germany.
livMatS@FIT (Freiburg Center for Interactive Materials and Bioinspired Technologies), University of Freiburg, Freiburg 79110, Germany.
ACS Appl Mater Interfaces. 2023 Dec 6;15(48):55346-55357. doi: 10.1021/acsami.3c12122. Epub 2023 Nov 20.
We present a new platform based on hydrogel beads for multiplex analysis that can be fabricated, barcoded, and functionalized in a single step using a simple microfluidic assembly and a photo-cross-linking process. The beads are generated in a two-phase flow fluidic system and photo-cross-linking of the polymer in the aqueous phase by C,H insertion cross-linking (CHic). The size and shape of the hydrogel particles can be controlled over a wide range by fluidic parameters. During the fabrication of the beads, they are barcoded by using physical and optical barcoding strategies. Magnetic beads and fluorescent particles, which allow identification of the production batch number, are added simultaneously as desired, resulting in complex, multifunctional beads in a one-step reaction. As an example of biofunctionalization, antigens were immobilized on the beads. Serum samples that originated from infected and non-infected patients could be clearly distinguished, and the sensitivity was as good as or even better than ELISA, the state of the art in clinical diagnostics. The ease of the one-step production process and the wide range of barcoding parameters offer strong advantages for multiplexed analytics in the life sciences and medical diagnostics.
我们提出了一种基于水凝胶珠的新平台，用于多重分析，可以通过简单的微流体制备、条码化和功能化一步完成，使用简单的微流体制备、光交联过程。珠子是在两相流流体系统中生成的，并且通过 C，H 插入交联（CHic）在水相中对聚合物进行光交联。通过流体参数可以在很宽的范围内控制水凝胶颗粒的大小和形状。在珠的制备过程中，使用物理和光学条码化策略对其进行条码化。根据需要，同时添加磁性珠和荧光颗粒，以识别生产批次号，从而在一步反应中得到复杂的多功能珠。作为生物功能化的一个例子，抗原被固定在珠上。可以清楚地区分来自感染和非感染患者的血清样本，并且灵敏度与临床诊断的最新技术 ELISA 一样好，甚至更好。一步生产工艺的简单性和广泛的条码化参数为生命科学和医学诊断中的多重分析提供了强大的优势。
