Xian Zhi-Hong, Cong Wen-Ming, Wang Yan-Hua, Wang Bin, Wu Meng-Chao
Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, PR China.
Pathol Res Pract. 2009;205(11):774-80. doi: 10.1016/j.prp.2009.06.014. Epub 2009 Jul 19.
We investigated the expression and localization of aquaporin-1 (AQP-1) in hepatitis B virus (HBV)-associated cirrhotic human liver tissues. The expression of AQP-1 at the protein and mRNA levels was analyzed by immunohistochemistry, quantitative real-time polymerase chain reaction (qPCR) and Western blotting in normal and HBV-associated cirrhotic human liver tissues. The correlation with the expression of CK19, CK7 and AQP-1 was also compared. AQP-1 staining was strongly and uniformly positive in mature bile ducts, isolated hepatic progenitor cells (HPCs) and ductular reactions. Scattered intermediate hepatocyte-like cells expressed AQP-1, which are often intimately associated with CK7 positive hepatocytes. However, the number of AQP-1+ intermediate hepatocyte-like cells was lower than that of CK7+ cells, and such positivity was rarely seen on stains for CK19. When compared with normal liver tissues, AQP-1 was overexpressed at both the mRNA and protein levels in the cirrhotic liver tissues. AQP-1 was overexpressed in the cirrhotic liver tissues. AQP-1, similar to CK19, might be a more specific and more sensitive marker than CK7 for the identification of HPCs.
我们研究了水通道蛋白-1（AQP-1）在乙型肝炎病毒（HBV）相关的肝硬化人肝组织中的表达及定位。通过免疫组织化学、定量实时聚合酶链反应（qPCR）和蛋白质印迹法分析正常及HBV相关的肝硬化人肝组织中AQP-1在蛋白质和mRNA水平的表达。还比较了其与细胞角蛋白19（CK19）、细胞角蛋白7（CK7）表达的相关性。AQP-1染色在成熟胆管、孤立的肝祖细胞（HPCs）和小胆管反应中呈强阳性且均匀一致。散在的中间型肝样细胞表达AQP-1，这些细胞常与CK7阳性肝细胞密切相关。然而，AQP-1阳性的中间型肝样细胞数量低于CK7阳性细胞，且在CK19染色中很少见这种阳性。与正常肝组织相比，AQP-1在肝硬化肝组织的mRNA和蛋白质水平均过度表达。AQP-1在肝硬化肝组织中过度表达。与CK19类似，AQP-1可能是比CK7更特异、更敏感的HPCs识别标志物。
