Sun C, Jin X-L, Xiao J-C
Department of Pathology, Ruijin Hospital, Shanghai Second Medical University, Shanghai, China.
Histopathology. 2006 Apr;48(5):546-55. doi: 10.1111/j.1365-2559.2006.02372.x.
It is still not clear whether oval cells demonstrate diverse morphology, immunophenotype or quantity in different human liver diseases. The aim of this study was to investigate these differences in hepatitis B virus (HBV)-positive and hepatitis C virus (HCV)-positive human liver cirrhosis (HLC).
Thirty-eight cases of HBV+ HLC and 32 cases of HCV+ HLC were investigated by light microscopy and immunohistochemistry for Hepatocyte, CK19, stem cell factor (SCF) and CD34. Five cases were also examined by transmission electron microscopy. Oval cells of similar morphology could be found in proliferating bile ductules in both groups. These cells coexpressed CK19 and Hepatocyte, but did not express SCF or CD34. Some of these cells exhibited a trend towards differentiation. There was no difference in the amount of oval cells between the two groups. The oval cell number was found to increase significantly with the progression of inflammation. A similar stem-like cell was not seen in the normal liver.
There are bipotential oval cells in both HBV+ and HCV+ HLC. The lack of difference in oval cells between the two groups suggests that they might play a similar biological role in the histogenesis of different liver diseases.
目前尚不清楚在不同的人类肝脏疾病中，卵圆细胞是否表现出不同的形态、免疫表型或数量。本研究的目的是调查乙型肝炎病毒（HBV）阳性和丙型肝炎病毒（HCV）阳性的人类肝硬化（HLC）中的这些差异。
通过光学显微镜和免疫组织化学对38例HBV+HLC和32例HCV+HLC进行研究，检测肝细胞、细胞角蛋白19（CK19）、干细胞因子（SCF）和CD34。对其中5例进行了透射电子显微镜检查。两组增生的胆小管中均可发现形态相似的卵圆细胞。这些细胞共表达CK19和肝细胞，但不表达SCF或CD34。其中一些细胞呈现出分化趋势。两组之间卵圆细胞的数量没有差异。发现卵圆细胞数量随着炎症进展而显著增加。在正常肝脏中未发现类似的干细胞样细胞。
HBV+HLC和HCV+HLC中均存在双潜能卵圆细胞。两组之间卵圆细胞缺乏差异表明它们可能在不同肝脏疾病的组织发生中发挥相似的生物学作用。
