Wu Jin-Bao, Han Yu-Jing, Nan Qing-Zhen, Zhang Zhen-Shu, Zhang Hong-Quan, Song Yu-Gang
Institute of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.:
Nan Fang Yi Ke Da Xue Xue Bao. 2009 Jul;29(7):1341-3.
To observe the effect of p21-activated kinase-1 (PAK1) gene transfection on the invasiveness of human colorectal carcinoma SW480 cells in vitro.
SW480 cells in routine culture were transfected with the recombinant plasmid EGFP-C1/PAK1 via Lipofectamine(TM) 2000. The expression of PAK1 protein in SW480 cells was detected using Western blotting, and the changes of the invasiveness of SW480 cells were evaluated using Boyden chamber invasion assay.
Forty-eight hours after transfection with pEGFP-C1/ PAK1, the PAK1 protein expression increased significantly in comparison with those in negative and vector control groups. The invasiveness of the SW480 cells was significantly enhanced after the transfection.
The PAK1 gene transfection can increase the expression of PAK1 in SW480 cells and enhance the invasiveness of the cells. PAK1 can be associated with the invasiveness and metastasis of colorectal carcinoma cells.
观察p21激活激酶-1（PAK1）基因转染对人结肠癌细胞系SW480体外侵袭能力的影响。
常规培养的SW480细胞采用脂质体2000介导重组质粒EGFP-C1/PAK1转染。采用蛋白质免疫印迹法检测SW480细胞中PAK1蛋白的表达，采用Boyden小室侵袭实验评估SW480细胞侵袭能力的变化。
转染pEGFP-C1/PAK1 48小时后，与阴性对照组和空载体对照组相比，SW480细胞中PAK1蛋白表达显著增加。转染后SW480细胞的侵袭能力显著增强。
PAK1基因转染可增加SW480细胞中PAK1的表达并增强细胞的侵袭能力。PAK1可能与结肠癌细胞的侵袭和转移有关。
