Namwong Sirilak, Tanasupawat Somboon, Lee Keun Chul, Lee Jung-Sook
Department of Biotechnology, Faculty of Science and Technology, Suan Sunandha Rajabhat University, Bangkok 10300, Thailand.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2254-9. doi: 10.1099/ijs.0.007161-0. Epub 2009 Jul 20.
A Gram-positive, rod-shaped, strictly aerobic, spore-forming, moderately halophilic bacterium, designated strain SSK2-2T, was isolated from fermented shrimp paste (ka-pi) produced in Thailand. It contained MK-7 as the predominant menaquinone and meso-diaminopimelic acid in the cell-wall peptidoglycan. The isolate grew at 8-43 degrees C, pH 6-9 and in 0.5-24% (w/v) NaCl (optimum, 6-14% NaCl). The major cellular fatty acids were anteiso-C15:0 and anteiso-C17:0. Phosphatidylglycerol and diphosphatidylglycerol were the major polar lipid components. The DNA G+C content was 39.7 mol%. Comparative 16S rRNA gene sequence analyses showed that strain SSK2-2T was most closely related to Oceanobacillus picturae KCTC 3821T with 98.7% sequence similarity. Based on phenotypic and molecular features combined with DNA-DNA hybridization results (<or=24.9% with O. picturae KCTC 3821T), this strain represents a novel species of the genus Oceanobacillus for which the name Oceanobacillus kapialis sp. nov. is proposed; the type strain is SSK2-2T (=KCTC 13177T=PCU 300T=TISTR 1858T).
从泰国生产的发酵虾酱（ka-pi）中分离出一株革兰氏阳性、杆状、严格需氧、产芽孢、中度嗜盐的细菌，命名为菌株SSK2-2T。它含有MK-7作为主要的甲基萘醌，细胞壁肽聚糖中含有内消旋二氨基庚二酸。该分离株在8-43℃、pH值6-9以及0.5-24%（w/v）NaCl（最适为6-14% NaCl）的条件下生长。主要的细胞脂肪酸为anteiso-C15:0和anteiso-C17:0。磷脂酰甘油和双磷脂酰甘油是主要的极性脂质成分。DNA的G+C含量为39.7 mol%。比较16S rRNA基因序列分析表明，菌株SSK2-2T与海洋芽孢杆菌（Oceanobacillus picturae）KCTC 3821T关系最为密切，序列相似性为98.7%。基于表型和分子特征以及DNA-DNA杂交结果（与海洋芽孢杆菌KCTC 3821T的杂交率≤24.9%），该菌株代表了海洋芽孢杆菌属的一个新物种，为此提出名为海洋虾酱芽孢杆菌（Oceanobacillus kapialis）的新种；模式菌株为SSK2-2T（=KCTC 13177T=PCU 300T=TISTR 1858T）。
