Lagier Jean-Christophe, Khelaifia Saber, Azhar Esam Ibraheem, Croce Olivier, Bibi Fehmida, Jiman-Fatani Asif Ahmad, Yasir Muhammad, Helaby Huda Ben, Robert Catherine, Fournier Pierre-Edouard, Raoult Didier
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, UM 63, CNRS 7278, L'Institut de Recherche pour le Développement 198, Inserm 1095, Institut Hospitalo-Universitaire Méditerranée-Infection, Faculté de Médecine, Aix-Marseille Université, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 5, France.
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Stand Genomic Sci. 2015 Oct 29;10:91. doi: 10.1186/s40793-015-0081-2. eCollection 2015.
Oceanobacillus picturae is a strain of a moderately halophilic bacterium, first isolated from a mural painting. We demonstrate, for the first time, the culture of human Oceanobacillus picturae, strain S1(T), whose genome is described here, from a stool sample collected from a 25-year-old Saoudian healthy individual. We used a slightly modified standard culture medium adding 100 g/L of NaCl. We provide a short description of this strain including its MALDI-TOF spectrum, the main identification tool currently used in clinical microbiology. The 3,675,175 bp long genome exhibits a G + C content of 39.15 % and contains 3666 protein-coding and 157 RNA genes. The draft genome sequence of Oceanobacillus picturae has a similar size to the Oceanobacillus kimchii (respectively 3.67 Mb versus 3.83 Mb). The G + C content was higher compared with Oceanobacillus kimchii (respectively 39.15 % and 35.2 %). Oceanobacillus picturae shared almost identical number of genes (3823 genes versus 3879 genes), with a similar ratio of genes per Mb (1041 genes/Mb versus 1012 genes/Mb). The genome sequencing of Oceanobacillus picturae strain S1 isolated for the first time in a human, will be added to the 778 genome projects from the gastrointestinal tract listed by the international consortium Human Microbiome Project.
海洋壁画芽孢杆菌是一种中度嗜盐细菌菌株，首次从一幅壁画中分离出来。我们首次展示了从一名25岁沙特健康个体的粪便样本中培养出的人源海洋壁画芽孢杆菌S1(T)菌株，此处描述了其基因组。我们使用了添加100 g/L氯化钠的略微改良的标准培养基。我们提供了该菌株的简短描述，包括其基质辅助激光解吸电离飞行时间质谱图谱，这是目前临床微生物学中使用的主要鉴定工具。该基因组长度为3,675,175 bp，G+C含量为39.15%，包含3666个蛋白质编码基因和157个RNA基因。海洋壁画芽孢杆菌的基因组草图序列大小与泡菜芽孢杆菌相似（分别为3.67 Mb和3.83 Mb）。与泡菜芽孢杆菌相比，其G+C含量更高（分别为39.15%和35.2%）。海洋壁画芽孢杆菌的基因数量几乎相同（3823个基因对3879个基因），每兆碱基的基因比例相似（1041个基因/Mb对1012个基因/Mb）。首次从人类中分离出的海洋壁画芽孢杆菌S1菌株的基因组测序，将被添加到国际人类微生物组计划列出的778个胃肠道基因组项目中。
