Amoozegar M A, Sánchez-Porro C, Rohban R, Hajighasemi M, Ventosa A
Extremophiles Laboratory, Department of Microbiology, Faculty of Biology, College of Science, University of Tehran, Tehran, Iran.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2352-8. doi: 10.1099/ijs.0.010090-0. Epub 2009 Jul 20.
A Gram-positive, moderately halophilic, endospore-forming bacterium, designated strain HS136T, was isolated from the hypersaline lake Howz-Soltan in Iran. Cells were motile rods, producing ellipsoidal endospores at a central-subterminal position in non-swollen sporangia. Strain HS136T, a strictly aerobic bacterium, grew between pH 7.0 and 10.0 (optimal growth at pH 8.0-8.5), between 25 and 45 degrees C (optimal growth at 40 degrees C) and at salinities of 5-20% (w/v) NaCl, growing optimally at 10% (w/v) NaCl. On the basis of 16S rRNA gene sequence analysis, strain HS136T was shown to belong to the genus Bacillus within the phylum Firmicutes and showed closest phylogenetic similarity to Bacillus salarius BH169T (95.2%) and Bacillus qingdaonensis CM1T (94.5%). The DNA G+C content of this new isolate was 37.1 mol%. The major cellular fatty acids of strain HS136T were iso-C15:0, anteiso-C15:0 and anteiso-C17:0, and its polar lipid pattern consisted of phosphatidylglycerol and diphosphatidylglycerol. The isoprenoid quinone was MK-7. The peptidoglycan type is A1gamma, with meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of polyphasic evidence from this study, Bacillus persepolensis sp. nov. is proposed, with strain HS136T (=CCM 7595T=DSM 21632T=JCM 15720T=LMG 25222T) as the type strain.
从伊朗的豪兹 - 索尔坦盐湖中分离出一株革兰氏阳性、中度嗜盐、产芽孢细菌，命名为HS136T菌株。细胞为能动杆菌，在非膨大孢子囊中于中央 - 亚末端位置产生椭圆形芽孢。HS136T菌株是严格需氧菌，在pH 7.0至10.0之间生长（最适生长pH为8.0 - 8.5），在25至45℃之间生长（最适生长温度为40℃），在5 - 20%（w/v）NaCl盐度下生长，最适生长盐度为10%（w/v）NaCl。基于16S rRNA基因序列分析，HS136T菌株被证明属于厚壁菌门芽孢杆菌属，与盐芽孢杆菌BH169T（95.2%）和青岛芽孢杆菌CM1T（94.5%）显示出最密切的系统发育相似性。该新分离株的DNA G + C含量为37.1 mol%。HS136T菌株的主要细胞脂肪酸为异C15:0、anteiso - C15:0和anteiso - C17:0，其极性脂质模式由磷脂酰甘油和双磷脂酰甘油组成。类异戊二烯醌为MK - 7。肽聚糖类型为A1γ，以中 - 二氨基庚二酸作为诊断性二氨基酸。基于本研究的多相证据，提出了新种波斯芽孢杆菌，以HS136T菌株（=CCM 7595T = DSM 21632T = JCM 15720T = LMG 25222T）作为模式菌株。
