Extremophiles Laboratory, Department of Microbiology, Faculty of Biology, College of Science, University of Tehran, Tehran, Iran.
Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR, Teheran, Iran.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):811-816. doi: 10.1099/ijs.0.030874-0. Epub 2011 May 13.
A Gram-positive, moderately halophilic rod, designated X5BT, was isolated from saline mud of the hypersaline lake Aran-Bidgol in Iran. Strain X5BT was a strictly aerobic, motile bacterium that produced ellipsoidal endospores at a central-subterminal position in non-swollen sporangia. The isolate grew at pH 7.0-10.0 (optimum pH 7.5), at 25-45 °C (optimum 35 °C) and with 2.5-15 % (w/v) NaCl (optimum 5-7.5 %). On the basis of 16S rRNA gene sequences, strain X5BT belonged to the genus Bacillus and showed highest similarity with Bacillus persepolensis HS136T (95.6 % 16S rRNA gene sequence similarity) and Bacillus salarius BH169T (95.5 %). The DNA G+C content was 42.4 mol%. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0 and the polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, three phospholipids and two glycolipids. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid and the isoprenoid quinones were MK-7 (92 %), MK-6 (6 %) and MK-5 (2 %). On the basis of phylogenetic, chemotaxonomic and phenotypic data, a novel species of the genus Bacillus is proposed, with the name Bacillus iranensis sp. nov. The type strain is X5BT (=IBRC 10446T=DSM 23995T).
一株革兰氏阳性、中度嗜盐的杆菌，命名为 X5BT，从伊朗高盐湖泊 Aran-Bidgol 的盐泥中分离得到。菌株 X5BT 是一种严格需氧、运动的细菌，在非肿胀的孢子囊中中央-亚末端位置产生椭圆形内生孢子。该分离物在 pH 7.0-10.0（最佳 pH 7.5）、25-45°C（最佳 35°C）和 2.5-15%（w/v）NaCl（最佳 5-7.5%）下生长。基于 16S rRNA 基因序列，菌株 X5BT 属于芽孢杆菌属，与 Bacillus persepolensis HS136T（95.6% 16S rRNA 基因序列相似性）和 Bacillus salarius BH169T（95.5%）最为相似。DNA G+C 含量为 42.4 mol%。主要细胞脂肪酸为 anteiso-C15:0 和 iso-C15:0，极性脂谱由磷脂酰甘油、双磷脂酰甘油、三种磷脂和两种糖脂组成。细胞壁肽聚糖中发现的二氨基氨基酸是 meso-二氨基庚二酸，异戊烯醌为 MK-7（92%）、MK-6（6%）和 MK-5（2%）。基于系统发育、化学分类学和表型数据，提出了芽孢杆菌属的一个新种，命名为 Bacillus iranensis sp. nov.。模式菌株为 X5BT（=IBRC 10446T=DSM 23995T）。
