Kumari Hansi, Gupta Sanjay Kumar, Jindal Swati, Katoch Parul, Lal Rup
Molecular Biology Laboratory, Department of Zoology, University of Delhi, Delhi 110007, India.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2291-6. doi: 10.1099/ijs.0.004739-0. Epub 2009 Jul 20.
The taxonomic position of a yellow-pigmented bacterial strain, designated DS20T, isolated from a hexachlorocyclohexane dump site at Lucknow, India was determined based on a polyphasic taxonomic characterization. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain DS20T occupied a distinct phylogenetic position in the Sphingobium cluster, showing highest similarity with 'Pseudomonas abikonensis' IAM 12404 (98.8%), followed by Sphingobium rhizovicinum CC-FH12-1T (97.4%) and Sphingobium olei IMMIB HF-1T (97.2%). Therefore, the taxonomic characterization of 'P. abikonensis' NBRC 16140 was also undertaken. Phylogenetic, chemotaxonomic and morphological analyses, based on signature sequences, DNA-DNA hybridizations, fatty acid profiles, physiological characterizations and polar lipid profiles confirmed that both strains DS20T and 'P. abikonensis' NBRC 16140 represent two distinct species of the genus Sphingobium. Therefore, two novel Sphingobium species are proposed, Sphingobium lactosutens sp. nov. (type strain, DS20T=CCM 7540T=MTCC 9471T) and Sphingobium abikonense sp. nov. (type strain, NBRC 16140T=IAM 12404T=KCTC 2864T).
从印度勒克瑙的六氯环己烷倾倒场分离出的一株黄色色素细菌菌株DS20T，通过多相分类学特征确定了其分类地位。基于16S rRNA基因序列构建的邻接树表明，菌株DS20T在鞘氨醇单胞菌属簇中占据独特的系统发育位置，与“阿比科假单胞菌”IAM 12404相似度最高（98.8%），其次是根际鞘氨醇单胞菌CC-FH12-1T（97.4%）和油鞘氨醇单胞菌IMMIB HF-1T（97.2%）。因此，还对“阿比科假单胞菌”NBRC 16140进行了分类学特征分析。基于特征序列、DNA-DNA杂交、脂肪酸谱、生理特征和极性脂质谱的系统发育、化学分类学和形态学分析证实，菌株DS20T和“阿比科假单胞菌”NBRC 16140均代表鞘氨醇单胞菌属的两个不同物种。因此，提出了两个新的鞘氨醇单胞菌物种，乳糖鞘氨醇单胞菌（Sphingobium lactosutens）新种（模式菌株，DS20T = CCM 7540T = MTCC 9471T）和阿比科鞘氨醇单胞菌（Sphingobium abikonense）新种（模式菌株，NBRC 16140T = IAM 12404T = KCTC 2864T）。
