Al Masalma Mouhamad, Raoult Didier, Roux Véronique
Laboratoire de Bactériologie-Virologie, Hôpital de la Timone, URMITE CNRS-IRD UMR6236, IFR48, 264 rue Saint-Pierre, 13385 Marseille, Cedex 05, France.
Int J Syst Evol Microbiol. 2009 Sep;59(Pt 9):2232-7. doi: 10.1099/ijs.0.007823-0. Epub 2009 Jul 20.
A strictly anaerobic bacterial strain, 7401987T, was isolated from a human brain abscess sample. Cells were Gram-negative, non-spore-forming, coccoid to rod-shaped and motile by flagella in a lophotrichous arrangement. The isolate was asaccharolytic and the major cellular fatty acids were anteiso-C15:0 (28.2%), C16:0 (18.0%), iso-C15:0 (12.3%) and iso-C17:0 3-OH (11.7%). 16S rRNA gene sequence comparisons showed that the isolate was distantly related to members of the genera Bacteroides (<83.6% similarity), Parabacteroides (<79.9% similarity), Tannerella (<79.8% similarity), Dysgonomonas (<79.6% similarity), Porphyromonas (<79.3% similarity) and Prevotella (<78.9% similarity). The low 16S rRNA gene sequence similarity values and physiological and biochemical characteristics differentiated strain 7401987T from all known species and indicate that our isolate represents a novel species in a new genus within the phylum Bacteroidetes. The name Phocaeicola abscessus gen. nov., sp. nov. is proposed; the type strain of Phocaeicola abscessus is 7401987T (=CCUG 55929T=CSUR P22T=DSM 21584T).
从一份人脑脓肿样本中分离出了一株严格厌氧的细菌菌株7401987T。细胞革兰氏阴性，不形成芽孢，呈球状至杆状，通过端生丛毛状排列的鞭毛运动。该分离株不分解糖类，主要细胞脂肪酸为anteiso-C15:0（28.2%）、C16:0（18.0%）、iso-C15:0（12.3%）和iso-C17:0 3-OH（11.7%）。16S rRNA基因序列比较表明，该分离株与拟杆菌属（<83.6%相似性）、副拟杆菌属（<79.9%相似性）、坦纳菌属（<79.8%相似性）、不良嗜菌属（<79.6%相似性）、卟啉单胞菌属（<79.3%相似性）和普雷沃菌属（<78.9%相似性）的成员亲缘关系较远。低16S rRNA基因序列相似性值以及生理生化特征将7401987T菌株与所有已知物种区分开来，表明我们的分离株代表了拟杆菌门一个新属中的一个新物种。提议将其命名为脑脓肿福卡菌属（Phocaeicola abscessus），新属，新种；脑脓肿福卡菌的模式菌株为7401987T（=CCUG 55929T=CSUR P22T=DSM 21584T）。
