Pardiñas López Simón, García-Caro Mónica E, Vallejo Juan A, Aja-Macaya Pablo, Conde-Pérez Kelly, Nión-Cabeza Paula, Khouly Ismael, Bou Germán, Cendal Ana Isabel Rodríguez, Díaz-Prado Silvia, Poza Margarita
Periodontology and Oral Surgery, Clínica Médico Dental Pardiñas, Real 66, 3, A Coruña, 15003, Spain.
Grupo de Terapia Celular y Medicina Regenerativa, Instituto de Investigación Biomédica de A Coruña (INIBIC), Servizo Galego de Saúde (SERGAS), Complexo Hospitalario Universitario de A Coruña (CHUAC), A Coruña, 15003, Spain.
Clin Oral Investig. 2025 Mar 14;29(4):182. doi: 10.1007/s00784-025-06267-8.
To evaluate the effect of coconut oil on the oral bacteriome and inflammatory response in patients with periodontitis by integrating next-generation sequencing analyses of pathogenic bacterial shifts and quantification of inflammatory markers, thereby assessing its potential as a natural adjunct to standard nonsurgical periodontal therapy.
A triple-blind clinical trial was conducted with 30 participants diagnosed with periodontitis, randomized into 3 groups: (1) coconut oil, (2) chlorhexidine and (3) placebo. Saliva and gingival crevicular fluid (GCF) samples were collected before treatment, one month after treatment, and one month post-non-surgical periodontal therapy. Bacterial DNA was extracted, and the V3-V4 region of the 16 S rRNA gene was PCR-amplified and sequenced using Illumina MiSeq technologies. Inflammatory biomarkers, including Interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), were quantified from GCF samples.
Coconut oil treatment significantly reduced pathogenic bacterial families such as Spirochaetaceae and Tannerellaceae while promoting beneficial bacteria such as Streptococcaceae. At the genus and species levels, coconut oil reduced pathogens such as Tannerella forsythia and Treponema denticola along with increase in beneficial bacteria such as Streptococcus. The subgingival microbial dysbiosis index improved significantly in both coconut oil and chlorhexidine groups. Furthermore, the coconut oil demonstrated a reduction in IL-6 and TNF-α levels, indicating decreased local inflammation.
Coconut oil treatment significantly modulated the oral microbiome and reduced inflammatory markers in patients with periodontitis, suggesting its potential as a natural and effective adjunct in periodontal therapy.
This study highlights coconut oil's potential as a natural adjunct in periodontal therapy, effectively reducing pathogenic bacteria and inflammatory markers (IL-6, TNF-α). It offers a safe alternative to chlorhexidine, promoting microbiome balance and improved periodontal health.
通过整合致病细菌变化的下一代测序分析和炎症标志物定量，评估椰子油对牙周炎患者口腔细菌群落和炎症反应的影响，从而评估其作为标准非手术牙周治疗天然辅助剂的潜力。
对30名被诊断为牙周炎的参与者进行了一项三盲临床试验，随机分为3组：（1）椰子油组，（2）氯己定组和（3）安慰剂组。在治疗前、治疗后1个月和非手术牙周治疗后1个月收集唾液和龈沟液（GCF）样本。提取细菌DNA，并使用Illumina MiSeq技术对16S rRNA基因的V3-V4区域进行PCR扩增和测序。从GCF样本中定量炎症生物标志物，包括白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）。
椰子油治疗显著减少了螺旋体科和坦纳菌科等致病细菌家族，同时促进了链球菌科等有益细菌。在属和种水平上，椰子油减少了福赛坦纳菌和具核梭杆菌等病原体，同时增加了链球菌等有益细菌。椰子油组和氯己定组的龈下微生物失调指数均显著改善。此外，椰子油使IL-6和TNF-α水平降低，表明局部炎症减轻。
椰子油治疗显著调节了牙周炎患者的口腔微生物群，并降低了炎症标志物，表明其作为牙周治疗天然有效辅助剂的潜力。
本研究强调了椰子油作为牙周治疗天然辅助剂的潜力，可有效减少致病细菌和炎症标志物（IL-6、TNF-α）。它为氯己定提供了一种安全的替代品，促进微生物群平衡并改善牙周健康。
