脊髓灰质炎病毒基因组蛋白在病毒RNA中的定位及其对RNA合成的影响。

The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.

作者信息

Nomoto A, Detjen B, Pozzatti R, Wimmer E

出版信息

Nature. 1977 Jul 21;268(5617):208-13. doi: 10.1038/268208a0.

PMID:196204

Abstract

Evidence is presented that a small protein (VPg) is covalently attached to the 5'-terminal oligonucleotide VPg-pU-U-A-A-A-A-C-A-Gp of the polio genome, to nascent strands of the polio replicative intermediate and to poly(U) of minus strands. A model of polio RNA replication is proposed implicating VPg in initiation of RNA symthesis, possibly as primer.

摘要

有证据表明，一种小蛋白（VPg）共价连接到脊髓灰质炎病毒基因组5'-末端寡核苷酸VPg-pU-U-A-A-A-A-C-A-Gp、脊髓灰质炎病毒复制中间体的新生链以及负链的多聚尿苷上。提出了一种脊髓灰质炎病毒RNA复制模型，认为VPg参与RNA合成的起始，可能作为引物。