Aly S S, Anderson R J, Whitlock R H, Fyock T L, McAdams S, Adaska J M, Jiang J, Gardner I A
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
J Dairy Sci. 2009 Aug;92(8):3634-42. doi: 10.3168/jds.2008-1680.
The reliability of environmental sampling to quantify Mycobacterium avium ssp. paratuberculosis (MAP) based on collector and time was evaluated. Fecal slurry samples were collected using a standardized protocol simultaneously by 2 collectors of different experience levels. Samples were collected from 30 cow pens on 4 dairies every other day on 3 occasions while cow movements between pens were minimal. The 4 study herds had moderate MAP seroprevalence and were housed in free-stall dairies in central California. Results of testing the environmental samples for MAP using PCR and culture were strongly correlated. The reliability of environmental sampling simultaneously by different collectors as estimated by the intraclass correlation coefficient was excellent (81%) for PCR and good (67%) for culture and may justify comparison of quantitative results of samples collected by different investigators. The reliability of environmental sampling over a 5-d period was good (67 and 64% for PCR and culture results, respectively), which justifies the utility of environmental sampling to identify pens with a high MAP bioburden between routine cow pen changes on a dairy. Environmental sampling of free-stall pens using the standardized sampling protocol yielded comparable PCR and culture results across collectors with different experience levels and at different times within a 5-d period.
基于采集者和时间评估了环境采样对定量鸟分枝杆菌副结核亚种（MAP）的可靠性。使用标准化方案由两名经验水平不同的采集者同时采集粪便浆样。在4个奶牛场的30个牛舍中，每隔一天在3个时间段采集样本，同时牛在牛舍间的移动极少。这4个研究牛群的MAP血清阳性率中等，饲养在加利福尼亚州中部的散栏式奶牛场。使用PCR和培养法检测环境样本中MAP的结果高度相关。通过组内相关系数估计，不同采集者同时进行环境采样的可靠性，PCR法极佳（81%），培养法良好（67%），这可能证明不同研究者采集样本的定量结果具有可比性。在5天期间进行环境采样的可靠性良好（PCR和培养结果分别为67%和64%），这证明了环境采样在奶牛场常规牛舍更换期间识别MAP生物负荷高的牛舍方面的实用性。使用标准化采样方案对散栏式牛舍进行环境采样，在5天期间内，不同经验水平的采集者以及不同时间的PCR和培养结果具有可比性。
