Goertz I, Baes C, Weimann C, Reinsch N, Erhardt G
Department of Animal Breeding and Genetics, Justus-Liebig-University, 35390 Giessen, Germany.
J Dairy Sci. 2009 Aug;92(8):4018-22. doi: 10.3168/jds.2008-1536.
In addition to its position in a quantitative trait locus for somatic cell score (SCS) and its function in the immune response, the CXCR1 chemokine receptor gene located on Bos taurus autosome 2 is an auspicious candidate gene for udder health in dairy cattle. An association analysis was conducted in the German Holstein-Friesian population to test the association between the single nucleotide polymorphism (SNP) at position +777 within the CXCR1 gene and the SNP at position -1768 in the 5' upstream region of this gene (rs41255711) and the indicator trait SCS by using a grand-daughter design. Data were analyzed for 3 families, with a total of 749 genotyped bulls and an extended pedigree of 8,071 animals from the national German bovine genome mapping project. Variance component estimation was used to determine the source of phenotypic variation in the chromosomal area of interest. A 20-cM window surrounding the 2 SNP in the CXCR1 gene was examined. Although the percentage of variance caused by the putative quantitative trait locus in the area studied was relatively large (12 and 11%, respectively), no statistically significant association of the SNP CXCR1+777 or CXCR1-1768 with SCS was found. These results contradict previously published studies.
位于牛2号常染色体上的CXCR1趋化因子受体基因，除了在体细胞评分（SCS）的数量性状基因座中具有定位以及在免疫反应中发挥作用外，还是奶牛乳房健康的一个理想候选基因。在德国荷斯坦 - 弗里生种群中进行了一项关联分析，通过孙女设计来测试CXCR1基因内第 +777 位的单核苷酸多态性（SNP）以及该基因5'上游区域第 -1768 位的SNP（rs41255711）与指示性状SCS之间的关联。对来自德国国家牛基因组图谱项目的3个家系的数据进行了分析，共有749头基因分型公牛以及8071头动物的扩展系谱。采用方差成分估计来确定感兴趣染色体区域内表型变异的来源。对CXCR1基因中2个SNP周围20厘摩的窗口进行了检查。尽管在所研究区域中假定的数量性状基因座引起的方差百分比相对较大（分别为12%和11%），但未发现SNP CXCR1+777或CXCR1-1768与SCS之间存在统计学上的显著关联。这些结果与先前发表的研究相矛盾。
