Visscher Marty O, Sullivan David, Sullivan Steven, Barford Brian, Dock Murray, Sommers Marilyn S
Cincinnati Children's Hospital Medical Center, The Skin Sciences Institute, Cincinnati, OH 45229, USA.
Skin Res Technol. 2009 May;15(2):180-6. doi: 10.1111/j.1600-0846.2008.00348.x.
BACKGROUND/PURPOSE: Epidermal injury is common but the accuracy of visual methods is significantly impacted by the inherent skin pigmentation. We examined imaging and fluorescence techniques to quantify tissue injury as a function of skin color.
Epidermal and mucosal scratches were created in 20 light- (L() 68.2+/-2.3) and 20 dark-skinned (L() 46.4+/-5.2) females. Injured and uninjured sites were treated with toluidine blue (TB), fluorescein (FL) and a TB/FL mixture and photographed under conditions of white and fluorescent light. Area and intensity parameters were determined.
Injured sites with TB and TB/FL had higher areas than the control for both light and dark subjects (ANOVA, P<0.05). The intensity of the injured TB site was higher than the control for light skin only. The areas of injured sites with FL and TB/FL were higher than the control for both groups as were the intensities of the injured sites with FL. The findings were similar for the lip skin.
Application of TB and FL contrasts under white and fluorescent light can be used to quantify tissue injuries for L(*) values >35 and is a promising approach for the quantitation across a range of skin pigmentation.
背景/目的：表皮损伤很常见，但视觉方法的准确性会受到皮肤固有色素沉着的显著影响。我们研究了成像和荧光技术，以将组织损伤量化为皮肤颜色的函数。
在20名浅色皮肤（L* 68.2±2.3）和20名深色皮肤（L* 46.4±5.2）女性中制造表皮和黏膜划痕。受伤和未受伤部位用甲苯胺蓝（TB）、荧光素（FL）和TB/FL混合物处理，并在白光和荧光条件下拍照。确定面积和强度参数。
对于浅色和深色受试者，使用TB和TB/FL处理的受伤部位面积均高于对照组（方差分析，P<0.05）。仅浅色皮肤的受伤TB部位强度高于对照组。两组中使用FL和TB/FL处理的受伤部位面积均高于对照组，使用FL处理的受伤部位强度也高于对照组。唇部皮肤的结果相似。
在白光和荧光下应用TB和FL对比可用于量化L*值>35时的组织损伤，是一种在一系列皮肤色素沉着范围内进行定量的有前景的方法。
