Chen Zhifei, Cheng Shaoting, Li Zibiao, Xu Kaitian, Chen Guo-Qiang
Multidisciplinary Research Center, Shantou University, Shantou, Guangdong 515063, People's Republic of China.
J Biomater Sci Polym Ed. 2009;20(10):1451-71. doi: 10.1163/092050609X12457419007621.
Novel tailor-made poly(ester urethane)s (PUs) based on microbial polyesters poly(3-hydroxybutyrate-co-4hydroxybutyrate) (P3HB4HB) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) were synthesized by melting polymerization (MP) using 1,6-hexamethylene diisocyanate (HDI) as a coupling agent. A comprehensive characterization using (1)H-NMR, Fourier transform infrared spectroscopy (FT-IR), gel-permeation chromatography (GPC), differential scanning calorimetry (DSC), mechanical properties, static water contact angles, cell proliferation using smooth muscle cells from rabbit aorta (RaSMCs) and immortalized human keratinocytes (HaCat), and blood coagulation behavior were conducted on the synthesized PUs films. DSC showed that PU samples had a low degree of crystallinity at room temperature and became fully amorphous after a melt-quenched process. The series of tailor-made PUs based on different mass ratios of P3HB4HB and PHBHHx revealed a ductile and flexile mechanical property especially for PHBHHx-rich PU, or a hydrophobic property for 4HB-rich PU. A 4 days incubation experiment showed that all PU films had a better cell proliferation than poly(lactic acid) (PLA), polyhydroxybutyrate (PHB), P3HB4HB and PHBHHx. RaSMCs cultured on PU films had a quiescent contractile phenotype, indicating that they were fully functional. HaCat incubated on tailor-made PU films showed a proliferation approximately equal to tissue-culture plates (TCPs). Blood coagulation behavior tests revealed a strong platelet adhesion and a short coagulation time on PU films. This study demonstrated potential medical applications for P3HB4HB and PHBHHx based polyurethane as a hydrophobic wound-healing and hemostatic materials.
基于微生物聚酯聚（3-羟基丁酸酯-co-4-羟基丁酸酯）（P3HB4HB）和聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）的新型定制聚（酯-聚氨酯）（PU）通过熔融聚合（MP）合成，使用1,6-六亚甲基二异氰酸酯（HDI）作为偶联剂。对合成的PU薄膜进行了全面表征，包括使用（1）H-NMR、傅里叶变换红外光谱（FT-IR）、凝胶渗透色谱（GPC）、差示扫描量热法（DSC）、力学性能、静态水接触角、使用兔主动脉平滑肌细胞（RaSMCs）和永生化人角质形成细胞（HaCat）的细胞增殖以及凝血行为。DSC表明，PU样品在室温下结晶度较低，经过熔体淬火过程后变为完全非晶态。基于不同质量比的P3HB4HB和PHBHHx的一系列定制PU显示出韧性和柔韧性的力学性能，特别是对于富含PHBHHx的PU，或者对于富含4HB的PU具有疏水性。为期4天的孵育实验表明，所有PU薄膜的细胞增殖都比聚乳酸（PLA）、聚羟基丁酸酯（PHB）、P3HB4HB和PHBHHx更好。在PU薄膜上培养的RaSMCs具有静止收缩表型，表明它们功能完全正常。在定制PU薄膜上孵育的HaCat显示出的增殖与组织培养板（TCPs）大致相当。凝血行为测试表明，PU薄膜上有强烈的血小板粘附和较短的凝血时间。本研究证明了基于P3HB4HB和PHBHHx的聚氨酯作为疏水性伤口愈合和止血材料的潜在医学应用。
