Qu Xiang-Hua, Wu Qiong, Liang Juan, Zou Bing, Chen Guo-Qiang
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China.
Biomaterials. 2006 May;27(15):2944-50. doi: 10.1016/j.biomaterials.2006.01.013. Epub 2006 Jan 26.
In this paper, comprehensive characteristics including cell attachment, cell proliferation status, cell cycle progression and phenotypic changes of smooth muscle cells from rabbit aorta (RaSMCs) were studied on poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) containing 0-20% HHx (mol%) in comparison with tissue culture plates (TCPs). Results demonstrated that RaSMCs adhered better on PHBHHx containing 12% HHx (12%HHx) although they proliferated better on 20%HHx-containing PHBHHx films (20%HHx). This was explained by the difference in cell cycle progression observed using flow cytometry, as it was found that only 20%HHx-containing polymer could maintain the normal cell cycle evolution as TCPs did after 3 d incubation. The highest expression level and typical spindle-like distribution of alpha-actin on 20%HHx-containing polymer were characterized as the contractile-like phenotype, suggesting that RaSMCs tended to differentiate rather than proliferate compared to the cells grown on 12%HHx polymer. Results obtained above suggested that 20%HHx was suitable for RaSMCs proliferation, leading to its change to contractile phenotype. This study extends the potential applications of PHBHHx in SMCs-related graft scaffold fabrication for tissue engineering.
在本文中，研究了兔主动脉平滑肌细胞（RaSMCs）在含0 - 20%己酸羟基丁酯（HHx，摩尔%）的聚（3-羟基丁酸酯-co-3-羟基己酸酯）（PHBHHx）上的综合特性，包括细胞附着、细胞增殖状态、细胞周期进程和表型变化，并与组织培养板（TCPs）进行了比较。结果表明，RaSMCs在含12% HHx（12%HHx）的PHBHHx上附着得更好，尽管它们在含20% HHx的PHBHHx膜（20%HHx）上增殖得更好。这可以通过流式细胞术观察到的细胞周期进程差异来解释，因为发现在孵育3天后，只有含20% HHx的聚合物能像TCPs一样维持正常的细胞周期演变。在含20% HHx的聚合物上，α-肌动蛋白的最高表达水平和典型的纺锤状分布被表征为收缩样表型，这表明与在含12% HHx聚合物上生长的细胞相比，RaSMCs倾向于分化而非增殖。上述结果表明，20% HHx适合RaSMCs增殖，导致其转变为收缩表型。本研究扩展了PHBHHx在组织工程中与平滑肌细胞相关的移植支架制造中的潜在应用。
