Liu Xue-Hua, Xia Hong, Zhou Xiu-Tian, Luo Wen, Zhou Jian-Guo, Dong Lin
Department of Oncology, The First Affiliated Hospital, University of South China, Hengyang, Hunan, 421001, PR China.
Ai Zheng. 2009 Apr;28(4):356-60.
Aloe has preventive effects on some chemotherapy-induced extravasation injuries. This study was to investigate the effect and mechanism of aloe gel on doxorubicin-induced extravasation injury.
Sprague-Dawley (SD) rats were used to establish the extravasation injury model induced by doxorubicin. Thirty SD rats were randomly divided into three groups: control group, aloe gel group (1 g/L) and 50% magnesium sulfate group. The area of extravasation was measured and the degree of injury was observed. The injured tissues were resected from two randomly selected rats in each group on the 1st, 4th, 7th, 11th, and 18th day after treatments. Pathological morphology of the resected tissues was observed under an optical microscope after hematoxylin and eosin (HE) staining. The exosmosis skin and subcutaneous tissues of rats were resected five days after treatments. Then the wounds were interruptedly sutured. When sutures were removed on the 7th day after operation, the condition of primary wound healing and the healing time were recorded. Expressions of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) in the exosmosis skin and subcutaneous tissues were detected by immunohistochemistry.
The area and the degree of extravasation injury were smaller and less severe in the aloe gel and magnesium sulfate groups than in the control group (P<0.01). The rates of primary wound healing were significantly higher in the aloe gel (60.0%) and magnesium sulfate (66.7%) groups than in the control group (20.0%); while the healing time was significantly shorter in the aloe gel (9.6+/-1.64 d) and magnesium sulfate (9.33+/-1.40 d) groups than in the control group (12.13+/-2.06 d) (both P<0.01). Moreover, the expression levels of VEGF and EGFR were higher in the aloe gel group than in the control group.
The preventive and therapeutic effects of aloe gel on doxorubicin-induced extravasation injury are satisfactory, which may be in relation to the up-regulation of VEGF and EGFR.
芦荟对某些化疗药物所致的外渗性损伤具有预防作用。本研究旨在探讨芦荟凝胶对多柔比星所致外渗性损伤的作用及机制。
采用斯普拉格-道利（SD）大鼠建立多柔比星所致的外渗性损伤模型。30只SD大鼠随机分为三组：对照组、芦荟凝胶组（1 g/L）和50%硫酸镁组。测量外渗面积并观察损伤程度。在治疗后第1、4、7、11和18天，从每组中随机选取2只大鼠切除损伤组织。苏木精-伊红（HE）染色后，在光学显微镜下观察切除组织的病理形态。治疗5天后切除大鼠外渗的皮肤和皮下组织，然后间断缝合伤口。术后第7天拆线时，记录伤口一期愈合情况及愈合时间。采用免疫组织化学法检测外渗皮肤和皮下组织中血管内皮生长因子（VEGF）和表皮生长因子受体（EGFR）的表达。
芦荟凝胶组和硫酸镁组的外渗损伤面积和程度均小于对照组，且损伤程度较轻（P<0.01）。芦荟凝胶组（60.0%）和硫酸镁组（66.7%）的伤口一期愈合率显著高于对照组（20.0%）；而芦荟凝胶组（9.6±1.64 d）和硫酸镁组（9.33±1.40 d）的愈合时间显著短于对照组（12.13±2.06 d）（均P<0.01）。此外，芦荟凝胶组VEGF和EGFR的表达水平高于对照组。
芦荟凝胶对多柔比星所致外渗性损伤的防治效果满意，其机制可能与上调VEGF和EGFR有关。
