Que Hua-Fa, Xing Jie
Institute of Chinese Traditional Surgery, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Zhong Xi Yi Jie He Xue Bao. 2008 Oct;6(10):1049-53. doi: 10.3736/jcim20081012.
To explore the effects of reinforcing qi and nourishing blood recipes on vascular endothelial growth factor (VEGF) and the microvessel count (MVC)in granulation tissue in rats with chronic skin ulcers.
A total of 90 male SD rats with back full-thickness skin lesion were used in this study. Except for the normal control group, the rats were injected with hydrocortisone for inducing chronic skin ulcers, and were randomly divided into untreated group, Sijunzi Decoction group, Siwu Decoction group and Bazhen Decoction group. The time of wound healing was observed; immunohistochemical method and image analytical method were used to test the expressions of VEGF and MVC in granulation tissue of dermal chronic ulcers in the rats.
In the untreated group, the time of wound healing was significantly longer than that in the normal group (P<0.01). The time of wound healing was obviously shortened in the three treated groups as compared with the untreated group (P<0.01, P<0.05). In the Sijunzi Decoction group, the time of wound healing was obviously shorter than those in Siwu Decoction group and Bazhen Decoction group (P<0.05, P<0.01). In the untreated group, the expression of VEGF and MVC were significantly lower than those in the normal control group (P<0.01). The expression of VEGF and MVC were obviously increased in the three treated groups as compared with those in the untreated group (P<0.01, P<0.05). In the Sijunzi Decoction group, the expression of VEGF and MVC were obviously lower than those in Siwu Decoction group and Bazhen Decoction group (P<0.05, P<0.01).
Reinforcing qi and nourishing blood recipes can promote wound healing in rats through up-regulating the expression of VEGF in granulation tissue of dermal chronic ulcers, and inducing angiogenesis.
探讨益气养血方剂对慢性皮肤溃疡大鼠创面肉芽组织中血管内皮生长因子（VEGF）及微血管计数（MVC）的影响。
选用90只背部全层皮肤损伤的雄性SD大鼠。除正常对照组外，其余大鼠注射氢化可的松诱导慢性皮肤溃疡，然后随机分为未治疗组、四君子汤组、四物汤组和八珍汤组。观察创面愈合时间；采用免疫组化法和图像分析法检测大鼠皮肤慢性溃疡创面肉芽组织中VEGF表达及MVC。
未治疗组创面愈合时间显著长于正常组（P<0.01）。与未治疗组比较，三个治疗组创面愈合时间明显缩短（P<0.01，P<0.05）。四君子汤组创面愈合时间明显短于四物汤组和八珍汤组（P<0.05，P<0.01）。未治疗组VEGF表达及MVC显著低于正常对照组（P<0.01）。与未治疗组比较，三个治疗组VEGF表达及MVC明显升高（P<0.01，P<0.05）。四君子汤组VEGF表达及MVC明显低于四物汤组和八珍汤组（P<0.05，P<0.01）。
益气养血方剂可通过上调皮肤慢性溃疡创面肉芽组织中VEGF表达，诱导血管生成，促进大鼠创面愈合。
