Arantes Tiago Pereira, Lopes Welber Daniel Zanetti, Ferreira Roberta Machado, Pieroni Juliana S Pinto, Pinto Vanessa M R, Sakamoto Claudio A, Costa Alvimar José da
CPPAR - Animal Health Research Center, UNESP, Jaboticabal, São Paulo, Brazil.
Exp Parasitol. 2009 Oct;123(2):190-4. doi: 10.1016/j.exppara.2009.07.003. Epub 2009 Jul 19.
Ten male dogs were distributed into three experimental groups for infection with Toxoplasma gondii: GI - three dogs inoculated with 2.0x10(5) P strais oocysts, GII - three dogs infected with 1.0x10(6) RH strain tachyzoites, and GIII - four controls dogs. Several clinical parameters were evaluated. IFAT was performed to detect anti-T. gondii antibodies. Presence of the parasite in semen was evaluated by PCR and bioassay techniques. Tissue parasitism was examined using bioassays and immunohistochemistry in testicle and epididymis fragments collected after orchiectomy. In semen samples collected from these two groups, the presence of T. gondii was verified by bioassays and PCR. T. gondii was detected by immunohistochemistry in tissues (testicle and epididymis fragments) of all six experimentally infected dogs. The T. gondii-positive seminal samples were used in the artificial insemination (AI) of four female dogs free of toxoplasmic infection. Seven days after AI, all of the female dogs presented serologic conversion (IFAT). Fetal reabsorption occurred in two of the dogs, while the others sustained full-term gestation. Several T. gondii cysts were detected in the brains of four offspring. These results suggest that T. gondii can be sexually transmitted in domestic dogs.
第一组（GI）——三只犬接种2.0×10⁵个P株卵囊；第二组（GII）——三只犬感染1.0×10⁶个RH株速殖子；第三组（GIII）——四只对照犬。评估了多个临床参数。采用间接荧光抗体试验（IFAT）检测抗刚地弓形虫抗体。通过聚合酶链反应（PCR）和生物测定技术评估精液中寄生虫的存在情况。在睾丸切除术后收集的睾丸和附睾组织碎片中，采用生物测定和免疫组织化学方法检查组织中的寄生虫感染情况。在从这两组收集的精液样本中，通过生物测定和PCR证实了刚地弓形虫的存在。在所有六只实验感染犬的组织（睾丸和附睾碎片）中，通过免疫组织化学检测到了刚地弓形虫。将刚地弓形虫阳性的精液样本用于对四只无弓形虫感染的雌性犬进行人工授精（AI）。人工授精后七天，所有雌性犬均出现血清学转化（IFAT）。两只犬发生了胎儿吸收，而其他犬维持了足月妊娠。在四只后代的大脑中检测到了多个刚地弓形虫囊肿。这些结果表明，刚地弓形虫可在家犬中通过性行为传播。
