伊朗 HIV 阳性患者中弓形虫、HBV 和 HCV 合并感染及其与 CD4 细胞的相关性。

Toxoplasma gondii, HBV, and HCV co-infection and their correlation with CD4 cells among Iranian HIV-positive patients.

机构信息

Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.

Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran.

出版信息

Immun Inflamm Dis. 2023 Feb;11(2):e794. doi: 10.1002/iid3.794.

DOI:10.1002/iid3.794

PMID:36840494

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9947625/

Abstract

INTRODUCTION

Human immunodeficiency virus (HIV/AIDS) infected patients have a higher risk of opportunistic infections (OIs) depending on their immunological status, especially CD4 + cell count. Toxoplasma gondii, hepatitis C virus (HCV), and hepatitis B virus (HBV) are important OIs among Human Immunodeficiency Virus (HIV)/Acquired Immune Deficiency Syndrome (AIDS) patients. However, little is known about co-infection of these pathogens among HIV-infected individuals and their correlation with the patient's CD4 + cell count. Hence, this study aimed to investigate the serological and molecular status of T. gondii infection among HIV-infected individuals who had co-infection with HBV and HCV infections.

METHODS

A total of 100 HIV/AIDS patients in two cities in the southwest of Iran was tested for T. gondii Immunoglobulin G (IgG) and Immunoglobulin M (IgM) antibodies as well as DNA detection by polymerase chain reaction (PCR) targeting the RE gene. HBV and HCV were detected by hepatitis B surface antigen (HBsAg) test, hepatitis C antibody (HCV Ab) test, and Real-Time PCR. The number of CD4 + cell counts was determined by Flow cytometry.

RESULTS

Anti-T. gondii IgG was positive in 22% of the patients, but anti-T. gondii IgM and PCR were negative in all samples. HBV and HCV were positive in 8% and 33% of the patients, respectively. Co-infections were as followed: HIV + HCV (16%), HIV + HCV + T. gondii (11%), HIV + T. gondii (5%), HIV + HBV (1%), HIV + HBV + T. gondii (1%), HIV + HBV + HCV (1%), and HIV + HBV + HCV + T. gondii (5%). A significant decline in CD4 + cell counts was found in such co-infection groups (HIV + T. gondii, HIV + HCV + T. gondii, and HIV + HBV + HCV + T. gondii) compared with the HIV mono-infection group.

CONCLUSIONS

Our study showed that co-infections of T. gondii, HCV, and HBV were common among HIV-infected patients and co-infections had a negative correlation with CD4 + cell counts of the patients.

摘要

简介

人类免疫缺陷病毒（HIV/AIDS）感染患者的机会性感染（OIs）风险较高，这取决于他们的免疫状态，尤其是 CD4+细胞计数。弓形体、丙型肝炎病毒（HCV）和乙型肝炎病毒（HBV）是 HIV/AIDS 患者中重要的 OIs。然而，对于 HIV 感染个体中这些病原体的合并感染以及它们与患者 CD4+细胞计数的相关性，人们知之甚少。因此，本研究旨在调查感染 HIV 的个体中弓形体感染的血清学和分子状态，这些个体同时合并感染 HBV 和 HCV。

方法

对伊朗西南部两个城市的 100 名 HIV/AIDS 患者进行了检测，以检测 IgG 和 IgM 抗体以及聚合酶链反应（PCR）针对 RE 基因的 DNA 检测。通过乙型肝炎表面抗原（HBsAg）检测、丙型肝炎抗体（HCV Ab）检测和实时 PCR 检测 HBV 和 HCV。通过流式细胞术确定 CD4+细胞计数。

结果

22%的患者抗弓形体 IgG 阳性，但所有样本的抗弓形体 IgM 和 PCR 均为阴性。HBV 和 HCV 的阳性率分别为 8%和 33%。合并感染如下：HIV+HCV（16%）、HIV+HCV+弓形体（11%）、HIV+弓形体（5%）、HIV+HBV（1%）、HIV+HBV+弓形体（1%）、HIV+HBV+HCV（1%）和 HIV+HBV+HCV+弓形体（5%）。与 HIV 单一感染组相比，在这些合并感染组（HIV+弓形体、HIV+HCV+弓形体和 HIV+HBV+HCV+弓形体）中发现 CD4+细胞计数显著下降。