Jun Kyung Ok, Song Chin-Hee, Kim Young-Bum, An Jinseok, Oh Jae Hyeun, Choi Sang Ki
Department of Biological Sciences, College of Life Industry and Science, Sunchon National University, Jeonnam, Republic of Korea.
FEBS Lett. 2009 Sep 3;583(17):2804-10. doi: 10.1016/j.febslet.2009.07.030. Epub 2009 Jul 19.
Previously we reported that in vitro translation activity in extracts of Saccharomyces cerevisiae was stimulated by dithiothreitol (DTT) and further increased by the addition of thioredoxin (TRX1) [Choi, S.K. (2007) Thioredoxin-mediated regulation of protein synthesis by redox in Saccharomyces cerevisiae. Kor. J. Microbiol. Biotechnol. 35, 36-40]. To identify the pathway affecting translation, we cloned and purified thioredoxin reductase 1 (TRR1), thioredoxin reductase 2 (TRR2), glutaredoxin 1 (GRX1) and glutaredoxin reductase 1 (GLR1) as fusion proteins. Thioredoxin-mediated activation of translation was more effectively stimulated by NADPH or NADH than by DTT. Moreover, addition of TRR1 led to a further increase of translation in the presence of thioredoxin plus NADPH. These findings indicate that redox control via the thioredoxin-thioredoxin reductase system plays an important role in the regulation of translation.
此前我们报道过，酿酒酵母提取物中的体外翻译活性受到二硫苏糖醇（DTT）的刺激，并且通过添加硫氧还蛋白（TRX1）进一步增强[崔，S.K.（2007年）硫氧还蛋白介导的酿酒酵母中氧化还原对蛋白质合成的调控。韩国微生物学与生物技术杂志。35，36 - 40]。为了确定影响翻译的途径，我们克隆并纯化了硫氧还蛋白还原酶1（TRR1）、硫氧还蛋白还原酶2（TRR2）、谷氧还蛋白1（GRX1）和谷氧还蛋白还原酶1（GLR1）作为融合蛋白。硫氧还蛋白介导的翻译激活被NADPH或NADH比被DTT更有效地刺激。此外，在存在硫氧还蛋白加NADPH的情况下，添加TRR1导致翻译进一步增加。这些发现表明，通过硫氧还蛋白 - 硫氧还蛋白还原酶系统的氧化还原控制在翻译调控中起重要作用。
