Kämpfer Peter, Busse Hans-Jürgen, Scholz Holger C
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2486-90. doi: 10.1099/ijs.0.008888-0. Epub 2009 Jul 21.
Two strains (LMG 3947 and LMG 3953) of motile, Gram-negative, violet-pigmented bacteria, respectively isolated in 1972 and before 1953 from environmental samples and previously assigned to Chromobacterium violaceum, were studied again in detail for their taxonomic position. 16S rRNA gene sequence analysis showed that both strains clustered together with the type strain of Chromobacterium violaceum (99.8 % sequence similarity for strain LMG 3953(T) and 98.9 % sequence similarity for LMG 3947(T)). recA sequence similarities were significantly lower (95-96 %), and fatty acid profiles were also different from those reported for C. violaceum and other Chromobacterium species. Both strains exhibit polar-lipid profiles consisting of the major compound phosphatidylethanolamine and moderate amounts of phosphatidylglycerol in addition to some minor lipids. The quinone systems consist of the major compound ubiquinone Q-8 and moderate amounts of Q-7. Polyamine patterns are composed of the major compounds putrescine and 2-hydroxyputrescine, moderate amounts of 1,3-diaminopropane and variable amounts of cadaverine and spermidine. The results of DNA-DNA hybridizations and physiological as well as biochemical tests allowed both genotypic and phenotypic differentiation of the two strains from described Chromobacterium species. It is evident from the genotypic and phenotypic data that both strains represent novel species in the genus Chromobacterium, for which we propose the names Chromobacterium piscinae sp. nov. (type strain LMG 3947(T) =CCM 3329(T)) and Chromobacterium pseudoviolaceum sp. nov. (type strain LMG 3953(T) =CCM 2076(T) =NCIMB [corrected] 8182(T)).
两株运动性革兰氏阴性、产紫色色素的细菌（LMG 3947和LMG 3953），分别于1972年和1953年之前从环境样本中分离得到，先前被归类为紫色色杆菌（Chromobacterium violaceum），现对其分类地位进行了详细的重新研究。16S rRNA基因序列分析表明，这两株菌与紫色色杆菌模式菌株聚类在一起（LMG 3953(T)的序列相似性为99.8%，LMG 3947(T)的序列相似性为98.9%）。recA序列相似性显著较低（95 - 96%），脂肪酸谱也与报道的紫色色杆菌及其他色杆菌属物种不同。这两株菌的极性脂质谱均由主要化合物磷脂酰乙醇胺和适量的磷脂酰甘油以及一些少量脂质组成。醌系统由主要化合物泛醌Q - 8和适量的Q - 7组成。多胺模式由主要化合物腐胺和2 - 羟基腐胺、适量的1,3 - 二氨基丙烷以及不同量的尸胺和亚精胺组成。DNA - DNA杂交以及生理和生化测试结果使得这两株菌在基因型和表型上与已描述色杆菌属物种有所区分。从基因型和表型数据可以明显看出，这两株菌代表了色杆菌属中的新物种，我们为此提议命名为水生色杆菌（Chromobacterium piscinae）新种（模式菌株LMG 3947(T) =CCM 3329(T)）和假紫色色杆菌（Chromobacterium pseudoviolaceum）新种（模式菌株LMG 3953(T) =CCM 2076(T) =NCIMB [更正后] 8182(T)）。
