Young Chiu-Chung, Lin Shi-Yao, Arun A B, Shen Fo-Ting, Chen Wen-Ming, Rekha P D, Langer Stefan, Busse Hans-Jürgen, Wu Yun-Hsuan, Kämpfer Peter
Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, Republic of China.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2909-15. doi: 10.1099/ijs.0.009415-0. Epub 2009 Jul 23.
A motile, Gram-negative, pinkish red-pigmented bacterium (strain CC-Hsuan-617(T)) was isolated from an oil-contaminated soil near an oil refinery located in Kaohsiung County, Taiwan. 16S rRNA gene sequence analysis showed that strain CC-Hsuan-617(T) clustered with Algoriphagus mannitolivorans (97.5 % sequence similarity), Algoriphagus aquatilis (98.0 %) and Algoriphagus boritolerans (97.5 %), followed by Algoriphagus ornithinivorans (97.1 %) and Algoriphagus alkaliphilus (97.1 %). The fatty acid profile of the novel strain was slightly different from those reported for recognized Algoriphagus species. The quinone system contained menaquinone MK-7 as the predominant component. The major polar lipids were phosphatidylethanolamine, an unknown polar lipid, an unknown phospholipid and an unknown aminophospholipid. The main cell polyamine was sym-homospermidine; lesser amounts of spermine and spermidine were also found. The results of DNA-DNA hybridization, and physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain CC-Hsuan-617(T) from recognized Algoriphagus species. Strain CC-Hsuan-617(T) is thus considered to represent a novel species of the genus Algoriphagus, for which the name Algoriphagus olei sp. nov. is proposed. The type strain is CC-Hsuan-617(T) (=BCRC 17886(T)=CCUG 57471(T)).
从台湾高雄县一家炼油厂附近受石油污染的土壤中分离出一株运动性、革兰氏阴性、呈粉红至红色色素的细菌（菌株CC-Hsuan-617(T)）。16S rRNA基因序列分析表明，菌株CC-Hsuan-617(T)与食甘露醇噬油菌（序列相似性97.5%）、水生噬油菌（98.0%）和耐硼噬油菌（97.5%）聚类在一起，其次是食鸟氨酸噬油菌（97.1%）和嗜碱噬油菌（97.1%）。该新菌株的脂肪酸谱与已确认的噬油菌属物种报道的脂肪酸谱略有不同。醌系统以甲基萘醌MK-7作为主要成分。主要极性脂为磷脂酰乙醇胺、一种未知极性脂、一种未知磷脂和一种未知氨基磷脂。主要的细胞多胺是共生高亚精胺；还发现了少量的精胺和亚精胺。DNA-DNA杂交以及生理生化试验的结果使得菌株CC-Hsuan-617(T)能够与已确认的噬油菌属物种在基因型和表型上区分开来。因此，菌株CC-Hsuan-617(T)被认为代表噬油菌属的一个新物种，为此提议将其命名为嗜油噬油菌（Algoriphagus olei sp. nov.）。模式菌株为CC-Hsuan-617(T)（=BCRC 17886(T)=CCUG 57471(T)）。
