在肺炎衣原体和肺炎支原体所致非典型呼吸道感染中同时使用直接和间接诊断技术。
Simultaneous use of direct and indirect diagnostic techniques in atypical respiratory infections from Chlamydophila pneumoniae and Mycoplasma pneumoniae.
作者信息
Pignanelli S, Shurdhi A, Delucca F, Donati M
机构信息
DESOS--Section of Microbiology, Policlinico S. Orsola, University of Bologna, 40138, Bologna, Italy.
出版信息
J Clin Lab Anal. 2009;23(4):206-9. doi: 10.1002/jcla.20332.
Abstract
In 2008, 50 samples (BAL), coming from hospital patients, with acute respiratory symptoms have been investigated using two real-time PCR methods: one assay for the single detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae DNA and one commercially available real-time duplex PCR assay for the detection of C. pneumoniae and M. pneumoniae DNA. Both techniques used here showed compliant results, with 100% concordance for detection of C. pneumoniae and 98% for detection of M. pneumoniae. The positive results obtained agreed with the clinical suspicion of such infections in some cases and with the presence of IgM specific for C. pneumoniae and M. pneumoniae in all cases of acute infection.
摘要
2008年，采用两种实时荧光定量聚合酶链反应（PCR）方法，对来自医院有急性呼吸道症状的50份患者支气管肺泡灌洗（BAL）样本进行了检测：一种用于单独检测肺炎衣原体和肺炎支原体DNA的检测方法，以及一种市售的用于检测肺炎衣原体和肺炎支原体DNA的实时荧光定量双重PCR检测方法。这里使用的两种技术结果一致，肺炎衣原体检测的一致性为100%，肺炎支原体检测的一致性为98%。在某些情况下，获得的阳性结果与这类感染的临床疑似情况相符，并且在所有急性感染病例中均与肺炎衣原体和肺炎支原体特异性IgM的存在情况相符。
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