通过实时聚合酶链反应检测和血清学检测分析肺炎衣原体和肺炎支原体呼吸道感染的儿童。
Analysis of children with Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae respiratoryinfections by real-time PCR assay and serological tests.
作者信息
Otomo Shinobu, Yamamura Jun-Ichi, Hayashi Erina, Nakamura Tsuneyuki, Kakinuma Hiroaki, Nakamoto Yumi, Takahashi Hiroaki, Karasawa Tadahiro
机构信息
Department of Clinical Laboratory Science, Division of Health Sciences, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
出版信息
APMIS. 2008 Jun;116(6):477-83. doi: 10.1111/j.1600-0463.2008.00973.x.
We examined 73 children with respiratory infections for Chlamydophila (Chlamydia) pneumoniae and Mycoplasma pneumoniae using real-time PCR assay and serological tests. C. pneumoniae and M. pneumoniae infections were found in 11 (15.1%) and 6 (8.2%) cases, respectively. The sensitivities and specificities of real-time PCR versus definite diagnosis of acute infection were 63.6% and 100% for C. pneumoniae, and 100% and 100% for M. pneumoniae, respectively. C. pneumoniae PCR-negative results appeared to be due to poor growth of the organism. The sensitivity and specificity of ImmunoCard tests were 33.3% and 82.1%, respectively, indicating that the efficacy of rapid diagnosis was disputable. The present results suggest that real-time PCR is suitable for rapid diagnosis as a first screening test to determine first-line antibacterial agents to be used against these infectious diseases.
我们使用实时聚合酶链反应（PCR）检测和血清学检测方法，对73例呼吸道感染儿童进行了肺炎衣原体和肺炎支原体检测。分别在11例（15.1%）和6例（8.2%）病例中发现了肺炎衣原体和肺炎支原体感染。实时PCR相对于急性感染确诊的敏感性和特异性，肺炎衣原体分别为63.6%和100%，肺炎支原体分别为100%和100%。肺炎衣原体PCR阴性结果似乎是由于该病原体生长不佳所致。免疫卡检测的敏感性和特异性分别为33.3%和82.1%，这表明快速诊断的效果存在争议。目前的结果表明，实时PCR作为一种初步筛查检测方法，适合用于快速诊断，以确定针对这些传染病的一线抗菌药物。
Zotero 插件