Fernández F, Costantini V, Barrandeguy M, Parreño V, Schiappacassi G, Maliandi F, Leunda M, Odeón A
Instituto Nacional de Tecnología Agropecuaria, Castelar, Pcia. de Buenos Aires, Argentina.
Rev Argent Microbiol. 2009 Apr-Jun;41(2):86-91.
The bovine viral diarrhea virus (BVDV) infection control should be based on elimination of persistently infected animals and on immunization through vaccination, to prevent fetal infection. However, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. This study evaluated the humoral immune response against homologous and heterologous strains of 7 inactivated BVDV vaccines, in bovines and two experimental models (ovine and guinea pig) which might be used to test candidate vaccines. Vaccines formulated with BVDV Singer, Oregon, NADL, and multivalent, induced seroconversion in the three animal models studied, reaching antibody titres higher than 2. Vaccine containing 125C -genotype 2- only induced a low antibody response in ovine, while VS-115 NCP vaccine was not immunogenic. Furthermore, bovine sera at 60 dpv presented homologous as well as heterologous antibody response, indicating a high degree of cross-reactivity among the strains studied. However, when bovine sera were tested against the Argentine field strain 00-693, they showed the lowest levels of cross-reactivity, suggesting the need of continued surveillance to identify and characterize emerging field BVDV strains. Finally, optimal correlations between bovine-ovine and bovine-guinea pig models were observed, indicating that two alternative species could replace bovines when testing the immunogenicity of BVDV candidate vaccines.
牛病毒性腹泻病毒（BVDV）感染的控制应基于清除持续感染的动物以及通过疫苗接种进行免疫，以防止胎儿感染。然而，由于其免疫原性较低，灭活BVDV疫苗的效力存在差异。本研究评估了7种灭活BVDV疫苗针对同源和异源毒株在牛以及两种可能用于测试候选疫苗的实验模型（绵羊和豚鼠）中的体液免疫反应。用BVDV Singer、Oregon、NADL以及多价疫苗配制的疫苗，在研究的三种动物模型中均诱导了血清转化，抗体滴度高于2。仅含125C -2型基因型的疫苗在绵羊中仅诱导了低水平的抗体反应，而VS-115 NCP疫苗没有免疫原性。此外，接种疫苗后60天的牛血清呈现出同源和异源抗体反应，表明所研究的毒株之间存在高度交叉反应性。然而，当用牛血清检测阿根廷田间毒株00 - 693时，它们表现出最低水平的交叉反应性，这表明需要持续监测以鉴定和表征新出现的田间BVDV毒株。最后，观察到牛 - 绵羊和牛 - 豚鼠模型之间具有最佳相关性，这表明在测试BVDV候选疫苗的免疫原性时，两种替代物种可以替代牛。
