Chai Junde, Ji Kangting, Lin Jiafeng, Ye Ruiyin, Tang Jifei, Yang Penglin
Department of Cardiology, the Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
Zhongguo Zhong Yao Za Zhi. 2009 Apr;34(7):904-7.
To observe the effects of danshensu on function of endothelial progenitor cells (EPCs) from peripheral blood which were damaged by oxidative low density lipoprotein (Ox-LDL). And study its possible mechanism.
Total mononuclear cells (MNCs) were isolated from peripheral blood by ficoll density gradient centrifugation, and were identified by demonstrating the expression of CD34, VEGFR-2 and AC133 with flow cytometry, to sure that all the cells needed were EPCs. Then the cells were plated on fibronectin-coated culture dishes. After incubation for 7 days, attached cells were collected and divided into three groups: Control group, Ox-LDL group, danshensu intervention group, stimulated with different cencentrations of danshensu (2, 10 and 50 mg x L(-1)), adhesion assay respectively. EPCs adhesion assay were performed by replating those on fibronectin-coated dishes, then adherent cells were counted. And take cell supernate of each group to carry on the SOD, MDA content examination.
Ox-LDL impaired EPC proliferative and adhesive capacity. In Ox-LDL group, The SOD content obviously drops, the MDA content obviously elevates. After danshensu interventing for 24 h, adhesive EPCs and migratory EPCs were significantly increased. Compared with Ox-LDL group, the SOD content of Danshensu intervention group obviously increased and the MDA content obviously reduced.
danshensu could improve proliferative and adhesive capacity of EPCs that were impaired by Ox-LDL. The mechanism might relate to the oxidation resistance damage.
观察丹参素对氧化型低密度脂蛋白（Ox-LDL）损伤的外周血内皮祖细胞（EPCs）功能的影响，并探讨其可能机制。
采用ficoll密度梯度离心法从外周血中分离单个核细胞（MNCs），通过流式细胞术检测CD34、VEGFR-2和AC133的表达进行鉴定，以确保所需细胞均为EPCs。然后将细胞接种于纤连蛋白包被的培养皿上。培养7天后，收集贴壁细胞并分为三组：对照组、Ox-LDL组、丹参素干预组，分别用不同浓度的丹参素（2、10和50mg·L⁻¹）刺激，进行黏附实验。通过将细胞重新接种于纤连蛋白包被的培养皿上进行EPCs黏附实验，然后计数贴壁细胞。并取各组细胞上清进行超氧化物歧化酶（SOD）、丙二醛（MDA）含量检测。
Ox-LDL损害EPCs的增殖和黏附能力。在Ox-LDL组中，SOD含量明显下降，MDA含量明显升高。丹参素干预24小时后，黏附的EPCs和迁移的EPCs显著增加。与Ox-LDL组相比，丹参素干预组的SOD含量明显增加，MDA含量明显降低。
丹参素可改善被Ox-LDL损伤的EPCs的增殖和黏附能力。其机制可能与抗氧化损伤有关。
