Whitehair K J, Parker J E, Smith G N, Adams S B, Bottoms G B
Department of Clinical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907.
Vet Surg. 1991 Sep-Oct;20(5):311-5. doi: 10.1111/j.1532-950x.1991.tb01273.x.
Serum levels of type III procollagen peptide (P-III-P) were measured by radioimmunoassay in clinically normal adult ponies (n = 15) and horses (n = 10). The mean serum levels of P-III-P from the ponies, 10.4 +/- 2.9 (SD) ng/mL, and the horses, 12.2 +/- 2.6 (SD) ng/mL, were not significantly different. Segments of jejunum were made ischemic to induce fibrous peritoneal adhesions in two ponies, and serum P-III-P levels were measured on days 4, 5, 7, 14, and 21. An exploratory celiotomy on day 21 revealed that the ischemic injury had induced fibrosis of the mesentery and bowel, but no adhesions had formed. The fibrotic mesentery contained type III collagen. The highest mean serum level of P-III-P, 23.0 +/- 3.5 (SD) ng/mL on day 7, was more than 4 SD above the mean from the normal ponies. There was a significant difference in the serum P-III-P levels in the ponies on days 0 (7.1 +/- 1.6 ng/mL) and 7 (23.0 +/- 3.5 ng/mL). Serum levels of P-III-P may be useful to study fibrosis associated with intestinal ischemia.
通过放射免疫分析法检测了临床正常的成年小马（n = 15）和成年马（n = 10）血清中III型前胶原肽（P-III-P）的水平。小马的P-III-P平均血清水平为10.4±2.9（标准差）ng/mL，马的为12.2±2.6（标准差）ng/mL，二者无显著差异。对两匹小马的空肠段进行缺血处理以诱导纤维性腹膜粘连，并在第4、5、7、14和21天测量血清P-III-P水平。第21天的剖腹探查显示，缺血性损伤已导致肠系膜和肠纤维化，但未形成粘连。纤维化的肠系膜含有III型胶原。第7天P-III-P的最高平均血清水平为23.0±3.5（标准差）ng/mL，比正常小马的平均值高出4个标准差以上。在第0天（7.1±1.6 ng/mL）和第7天（23.0±3.5 ng/mL），小马的血清P-III-P水平存在显著差异。血清P-III-P水平可能有助于研究与肠道缺血相关的纤维化。
