Oh Yong-Sik, Lim Hayung-Joon, Cha In-Tae, Im Wan-Taek, Yoo Jae-Soo, Kang Ui Gum, Rhee Sung-Keun, Roh Dong-Hyun
Department of Microbiology and Biotechnology Research Institute, Chungbuk National University, Cheongju 361-763, Chungbuk, Republic of Korea.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2718-23. doi: 10.1099/ijs.0.002576-0. Epub 2009 Jul 22.
A Gram-reaction-negative, non-motile, aerobic bacterium, designated HJ50(T), was isolated from deep seawater of the East Sea, South Korea. Cells were ovoid to rod-shaped (0.5-0.8x1.3-3.0 mum), often with unequal ends, suggesting a budding mode of reproduction. The strain had an absolute requirement for sea salts and tolerated up to 20 % (w/v) sea salts. Propionate, dl-lactate, 2-ketogluconate, 3-hydroxybutyrate and rhamnose were used as growth substrates, but not mannitol, salicin, 4-hydroxybenzoate or acetate. The major fatty acid was summed feature 7 (C(18 : 1)omega7c/omega9t/omega12t) and the DNA G+C content was 59.0+/-0.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that this strain was affiliated with the genus Roseovarius. Similarities between the 16S rRNA gene sequence of strain HJ50(T) (1430 nt) and those of type strains of members of the genus Roseovarius were 94.1-96.3 %. DNA-DNA relatedness values between strain HJ50(T) and the type strains of members of the genus Roseovarius were low (1.3-24.6 %). Physiological and biochemical differences support assignment of strain HJ50(T) to the genus Roseovarius as a representative of a novel species. The name Roseovarius halotolerans sp. nov. is proposed, with HJ50(T) (=KCTC 22224(T) =LMG 24468(T)) as the type strain.
从韩国东海深层海水中分离出一株革兰氏阴性、无运动性、需氧细菌，命名为HJ50(T)。细胞呈卵形至杆状（0.5 - 0.8×1.3 - 3.0μm），两端常不等，提示为出芽繁殖方式。该菌株对海盐有绝对需求，能耐受高达20%（w/v）的海盐。丙酸盐、dl - 乳酸盐、2 - 酮基葡萄糖酸盐、3 - 羟基丁酸盐和鼠李糖用作生长底物，但甘露醇、水杨苷、4 - 羟基苯甲酸盐或乙酸盐不能利用。主要脂肪酸为总和特征7（C(18 : 1)ω7c/ω9t/ω12t），DNA G + C含量为59.0±0.1 mol%。基于16S rRNA基因序列的系统发育分析表明，该菌株属于玫瑰色杆菌属。菌株HJ50(T)（1430 nt）的16S rRNA基因序列与玫瑰色杆菌属成员模式菌株的序列相似性为94.1 - 96.3%。菌株HJ50(T)与玫瑰色杆菌属成员模式菌株之间的DNA - DNA相关性值较低（1.3 - 24.6%）。生理生化差异支持将菌株HJ50(T)归为玫瑰色杆菌属的一个新物种代表。提出新物种名称耐盐玫瑰色杆菌（Roseovarius halotolerans sp. nov.），以HJ50(T)（=KCTC 22224(T) =LMG 24468(T)）作为模式菌株。
